THE EXPRESSION AND FUNCTION OF IPLA2B IN B-CELLS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 许多细胞通过磷脂酶A2表达一个组，命名为iPLA2B，它不需要钙来激活，受ATP刺激，对溴烯醇内酯自杀底物(BEL)的抑制敏感。对不同细胞系统的研究表明，iPLA2B在磷脂重塑、信号转导、细胞增殖和细胞凋亡中发挥作用。我们发现，胰岛、B细胞和葡萄糖反应性胰岛素瘤细胞表达iPLA2B，它参与葡萄糖刺激的胰岛素分泌，但不参与膜磷脂重塑。此外，最近的研究表明，iPLA2B参与了促进B细胞增殖和细胞凋亡的途径，并且各种磷脂衍生的介质参与了这些过程。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Many cells express a Group VIA phospholipase A2, designated iPLA2B, that does not require calcium for activation, is stimulated by ATP, and is sensitive to inhibition by a bromoenol lactone suicide substrate (BEL). Studies in various cell systems have led to the suggestion that iPLA2B has a role in phospholipids remodeling, signal transduction, cell proliferation, and apoptosis. We have found that pancreatic islets, B-cells, and glucose-responsive insulinoma cells express an iPLA2B that participates in glucose-stimulated insulin secretion but is not involved in membrane phospholipids remodeling. Additionally, recent studies reveal that iPLA2B is involved in pathways that contribute to B-cell proliferation and apaptosis, and that various phospholipid-derived mediators are involved in these processes.