REGULATION OF CYTOPLASMIC DYNEIN BASED VESICLE TRANSPORT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 动力蛋白是一种多亚单位蛋白质复合体，它与微管为基础的马达(细胞质动力蛋白)一起工作，提供广泛的亚细胞定位和运动功能。动力蛋白通过增强运动过程和作为适配器蛋白，使动力蛋白与各种亚细胞物质相互作用，从而帮助动力蛋白。要做到这一点，dynactin依赖于其结构中不同元素的不同结合活性。货物结合结构域和动力蛋白结合结构域允许动力蛋白作为动力蛋白适配器，而加工能力的增强依赖于微管结合结构域和动力蛋白结合结构域。所有已知的基于动力蛋白的细胞质转运都需要动力蛋白的结构完整性。动力蛋白亚基Dynamitin(P50)是一种分子“粘合剂”，它将动力蛋白和微管结合亚基P150(粘合)固定在货物结合元件上。在动力蛋白内部，动力蛋白与至少三个其他动力蛋白亚基以及自身接触。该项目致力于获得关于动力蛋白亚基组织、动力蛋白亚基修饰以及确定动力蛋白与细胞内相互作用的蛋白质的新信息。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dynactin is a multisubunit protein complex that works in conjunction with the microtubule-based motor, cytoplasmic dynein, to provide a wide range of subcellular localization and motile functions. Dynactin assists dynein by enhancing motor processivity and serving as an adaptor protein that allows dynein to interact with a variety of subcellular cargoes. To do this, dynactin relies on the distinct binding activities of different elements of its structure. The cargo-binding and dynein-binding domains allow dynactin to serve as a dynein adaptor, while processivity enhancement depends on the microtubule-binding and dynein-binding domains. All known cytoplasmic dynein-based transport requires dynactin's structural integrity. The dynactin subunit dynamitin (p50) acts as a molecular "glue" that anchors the dynein and microtubule-binding subunit of dynactin, p150(Glued), to the cargo-binding element. Within dynactin, dynamitin makes contacts with at least three other dynactin subunits as well as itself. This project is focused on obtaining new information on dynactin subunit organization, modification of dynactin subunits, and identifying proteins with which dynactin interacts in cells.