HOST INTERACTIONS OF THE YEAST RETROTRANSPOSON TY3

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Ty3产生的病毒样颗粒类似于逆转录病毒的核心颗粒。然而，在Ty3的情况下，结构蛋白开放阅读框架中约60个Ala扫描突变可用于研究结构蛋白与其自身以及与宿主蛋白的相互作用。我们已经构建了两个针对Gal4介导的控制的混合诱饵和激活载体中的Gag3插入。结构蛋白(Gag3)已被证明与自身相互作用，但在某些突变体中会受到影响。其中两个突变是影响Gag3和宿主蛋白之间相互作用的候选基因。初步数据表明，这些蛋白可能参与囊泡运输和P小体定位。我们建议筛选酵母基因组文库，以测试与Ty3 Gag3相互作用并参与颗粒组装的宿主蛋白。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ty3 produces virus-like particles similar to the core particles of retroviruses. In the case of Ty3 however, about 60 Ala scanning mutations in the structural protein open reading frame are available with which to study interactions of the structural protein with itself and with host proteins. We have constructed Gag3 insertions in two hybrid bait and activation vectors which target Gal4-mediated control. The structural protein (Gag3) has been shown to interact with itself but to be affected in certain mutants. Two of the mutations are candidates for affecting interactions between Gag3 and host proteins. Preliminary data suggests that these proteins might be involved in vesicular trafficking and P-body localization. We propose to screen a yeast genomic library in order to test for host proteins that interact with Ty3 Gag3 and are involved in particle assembly.