CHARACTERIZATION OF TRICHOMONAD LIPOPHOSPHOGLYCAN

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 毛滴虫、毛滴虫T. vagelis（Tv）和T.胎儿（Tf）分别引起人和牛主要性传播疾病。BNS等人从两种寄生虫中纯化脂磷酸聚糖，并用弱酸（三氟乙酸）和不同的酶（内切β-半乳糖苷酶、另一种β-半乳糖苷酶、磷脂酶C）处理，以获得足够短的寡糖结构，用于MS/MS表征。首先通过MALDI-TOF MS对Tv的馏分进行表征，然后将寡糖部分还原并全甲基化，然后使用石墨柱和QoTOF质谱仪通过LC-MS/MS进行分析。鉴定了分配给不同结构基序的各种糖。数据解释和调查正在进行中。生物学特性表明，T.脂磷酸聚糖触发人类女性生殖道上皮细胞选择性上调细胞因子。描述这些结果的手稿已提交出版。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Trichomonad parasites, T. vaginalis (Tv) and T. foetus (Tf), cause major sexually transmitted diseases in human and cattle, respectively. BNS et al. purified lipophosphoglycan from both parasites an treated them with mild acid (trifluoroacetic acid) and different enzymes (endo-beta-galactosidase, another beta-galactosidase, phospholipase C) in order to obtain oligosaccharide structures short enough for MS/MS characterization. The fractions from Tv were initially characterized by MALDI-TOF MS, then the oligosaccharide portions were reduced and permethylated before analysis by LC-MS/MS, using a graphite column and a QoTOF mass spectrometer. A variety of sugars assigned to different structural motifs was identified. The data interpretation and investigation is ongoing. Biological characterization has shown that the T. vaginalis Lipophosphoglycan triggers a selective upregulation of cytokines by human female reproductive tract epithelial cells. A manuscript describing these results has been submitted for publication.