ESI QQQ FTMS DEVELOPMENT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 由MDS Sciex提供的三重四极电喷雾电离源已经与自制的傅里叶变换质谱仪集成在一起。该系统允许低丰度翻译后修饰多肽的选择性积累，所选离子的Q2-CAD，自上而下的蛋白质测序，以及ICR细胞中的ECD/Sori-CAD/IRMPD。这些多重碎裂方法被用来获得具有高分辨率FTMS数据的肽序列信息。亚毫微克分子灵敏度和高转移效率是可能的。 已经设计了对该仪器的进一步升级，并已准备好安装。这些升级包括安装用于支撑和对准所有离子光学元件和ICR电池的轨道系统，以及安装一个新的电池，该电池将增加每次实验期间可被困在电池内的质量范围，并增加电池的容量。ECD喷枪的放置和安装也将进行改进，以减少喷枪产生的热量对ICR单元的影响，并允许为IRMPD引入来自二氧化碳激光器的光束。 对仪器进行了升级，包括加热金属毛细管和油雾过滤器-这提高了仪器的性能和稳定性。该仪器现在有95%的正常运行时间，并正在为许多协作项目生成数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A triple quadrupole electrospray ionization source, provided by MDS Sciex, has been integrated with a home built Fourier transform mass spectrometer. This system allows selected accumulation of low abundance post-translationally modified peptides, Q2-CAD of selected ions, top-down protein sequencing, and ECD/SORI-CAD/IRMPD in the ICR cell. These multiple fragmentation methods are used to obtain peptide sequence information with high resolution FTMS data. Sub-femtomole sensitivity and high transfer efficiency are possible. Further upgrades to the instrument have been designed and are ready for installation. These upgrades include the installation of a rail system for the support and alignment of all ion-optics and the ICR cell and the installation of a new cell that will increase the range of masses that can be trapped in the cell during each experiment as well as increase the capacity of the cell. The placement and mounting of the ECD gun will also be modified to reduce the impact of heat generated by the gun on the ICR cell and to allow the introduction of the beam from a CO2 laser for IRMPD. Upgrades have been added to the instrument including a heated metal capillary and oil mist filters - which have improved performance and stability of the instrument. The instrument now has 95% uptime and is generating data for a number of collaborative projects.