ES CELL CULTURE PROGRAM: MOUSE ES CELLS: GLYCOSYLATION PATTERNS OF GLYCOLIPIDS

这个子项目是众多研究子项目之一

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the present study we are characterizing the lectin binding properties of mouse embryonic stem cells and describing changes in binding that occur during differentiation. This analysis is being extended to the analysis of glycolipids and their glycosylation under the same conditions. One major objective of this work is to define cell surface epitopes on the surface of mESCs that can improve the analysis of the pluripotent state and to improve the analysis of early differentiation events. Current cell surface markers used to define mESCs and their differentiated derivatives have limited utility as their expression is not tightly restricted to a particular cell state and because changes in their presentation show poor temporal resolution in relation to differentiation events.