STRUCTURAL STUDIES OF THE RNA INTERFERENCE MACHINERY

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 RNA干扰（RNAi）是真核生物基因沉默和调控的一种保守机制。在人类中，RNAi和相关的micro-RNA途径由一个大的相关蛋白质复合物家族进行，一般称为RNA诱导沉默复合物（RISC）。这项工作的目标是确定各种形式的RISC的分子结构，以更好地理解RNAi基因沉默的机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA interference (RNAi) is a conserved mechanism of eukaryotic gene silencing and regulation. In humans RNAi and the related micro-RNA pathways are carried out by a family of large, related protein complexes, generically termed RNA induced silencing complexes (RISCs). The goal of this work is to determine molecular structures of the various forms of RISC in an effort to better understand the mechanism of gene silencing by RNAi.