COMPARATIVE PROTEOMIC INVESTIGATION OF ARDS AND SYSTEMIC INFLAMMATORY INJURY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 急性呼吸窘迫综合征（ARDS）是重症监护医学中发病和死亡的重要原因之一。它可能是败血症、胃内容物吸入、肺炎或创伤等疾病的后遗症。它的特征是炎症反应，导致肺泡-毛细血管屏障的破坏，导致液体和蛋白质从血液流入肺泡腔。炎症反应的确切机制仍不完全清楚。已经进行了许多临床和实验性试验，以提高对可能的治疗方案的理解和评估。临床研究表明，机械通气的模式，即所使用的潮气量，影响患有这种疾病的患者的生存率。 我们研究的重点是三个不同的方面： - 急性呼吸窘迫综合征患者肺水肿液标本与对照组比较 - 大鼠呼吸机肺损伤的诱导及其与非通气对照组肺泡II型细胞蛋白质组的比较 - 通过缺血-再灌注诱导大鼠肝损伤和评估肺泡II型细胞的蛋白质组以研究全身炎症反应对这些细胞蛋白质组的影响 - 在与加州大学旧金山分校肝脏中心合作的另一个项目中，研究了炎症细胞的蛋白质组变化。这是使用分离的枯否细胞从大鼠缺血再灌注损伤。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The acute respiratory distress syndrome (ARDS) is one of the most important causes for morbidity and mortality in intensive care medicine. It can be the sequel of diseases like sepsis, aspiration of gastric contents, pneumonia or trauma. It is characterized by an inflammatory reaction that leads to a breakdown of the alveolar-capillary barrier, resulting in an influx of fluid and proteins from the blood into the alveolar space. The exact mechanism of the inflammatory reaction is still incompletely understood. Numerous clinical and experimental trials have been made in order to improve the understanding and evaluate possible treatment options. It has been shown in clinical studies that the mode of mechanical ventilation, namely the tidal volume that is used, impacts survival of patients with this disease. The focus of our studies was on three different aspects: - The evaluation of pulmonary edema fluid samples of patients with ARDS compared to control samples - Induction of ventilator induced lung injury in rats and comparison of the proteome of alveolar type II cells from these animals with cells from not ventilated control animals - Induction of liver damage in rats by ischemia-reperfusion and evaluation of the proteome of the pulmonary alveolar type II cells to investigate the influence of a systemic inflammatory response on the proteome of these cells - In an additional project in cooperation with the UCSF liver center, proteomic changes in inflammatory cells were investigated. This was done using isolated Kupffer cells from rats with ischemia-reperfusion injury.