IMAGING OF PRE-NEOPLASTIC LESIONS IN A MOUSE MODEL OF MEDULLOBLASTOMA

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们实验的目的是在斑贴突变小鼠（人类髓母细胞瘤模型）中对肿瘤前病变进行成像。我们以前的研究表明，75-85%的这些动物的小脑表面有肿瘤前细胞，尽管只有15%会发展成完全的肿瘤。我们感兴趣的是确定这些病变的大小，形状和患病率，并跟踪它们以确定 当他们没有成为肿瘤。这些研究将分两个阶段进行。第一阶段：磁共振组织学，以确定肿瘤前病变的特点和灵敏度和分辨率，他们可以检测。这将涉及灌注后4-6只小鼠的高分辨率成像。第二阶段。4-6只动物的连续体内成像 跟踪肿瘤前病变的消失和/或这些病变发展成肿瘤。第二阶段的可行性将在第一阶段确定。这些研究将为髓母细胞瘤的肿瘤进展机制提供重要的见解。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of our experiments is to image pre-neoplastic lesions in patched mutant mice, a model for human medulloblastoma. Our previous studies indicate that 75-85% of these animals have pre-neoplastic cells on the surface of their cerebellum, although only 15% will go on to develop full-blown tumors. We are interested in determining the size, shape and prevalence of these lesions, and in tracking them to determine what happens to them when they do not become tumors. These studies will involve two phases. Phase I: MR histology to determine the characteristics of pre-neoplastic lesions and the sensitivity and resolution with which they can be detected. This will involve high-resolution imaging of 4-6 mice after perfusion. Phase II. Serial in vivo imaging of 4-6 animals to track the disappearance of pre-neoplastic lesions and/or the development of these lesions into tumors. The feasibility of Phase II will be determined during Phase I. Together these studies will provide important insight into the mechanisms of tumor progression in medulloblastoma.