INVENTION DISCLOSURES - CENTER FOR IN VIVO MICROSCOPY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在本报告期内，向杜克科学技术办公室提交了2项发明披露： 12/2007：Cristian T.作者：Badea，Samuel约翰斯顿，G.艾伦约翰逊：一次层析重建中多成像链投影图像组合的几何校正方法 本文首次提出了适用于双像素或多像素显微CT系统的几何校正方法。相同的技术可以用于两个以上的成像链。我们不知道其他出版的关于这个问题的工作。由于我们的方法涉及每个单独链条的几何校准，我们只能比较 我们的部分结果与以前的工作5.虽然我们部分使用与Yang等人相同的公式。5来初步估计五个几何参数，但由于基于优化步骤的额外细化，我们的单链方法显示出优势。基于优化的改进提高了重建的质量。我们的研究结果表明，我们也找到了准确和精确的值的双系统参数，包括<$z和a。 12/2007：G.艾伦约翰逊，博士，Gabriel Howles-Banerji、Kristin Frinkley、Kathy Nightingale博士Mark Palmeri，医学博士，博士学位： 脑内活体MR成像的活性染色开发、实施并测试了开放血脑屏障和活性染色的方法。提供了对超声波方法机理的见解。 我们描述了一种用于主动染色以增强活体动物脑中的MR信号的方法，即，体内对比增强脑成像。主动染色方法可用于提供空间分辨率的实质性增加比以前看到的大5倍以上。相同的方法可以与特定的探针和实验方案一起使用，以提供活体啮齿动物中神经元活动的定量定位测量：小鼠或大鼠脑的功能性MR成像，再次以比先前已经证明的大得多的空间分辨率进行。最后，通过合适的探针和协议，该方法有望帮助定义大脑中特定分子靶点的密度，并结合非常高分辨率的3D体内解剖定义。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This reporting period, 2 invention disclosures were submitted to the Duke Office of Science and Technology: 12/2007: Cristian T. Badea, Samuel Johnston, G. Allan Johnson: Geometric Calibration Procedure for the Combination of Projection Images Acquired with Multiple Imaging Chains in a Single Tomographic Reconstruction We have presented here the first geometric calibration method suitable for a dual or multipel micro-CT system. The same technique can be used for more than two imaging chains. We are not aware of other published prior work on this subject. Since our method involves the geometric calibration of each individual chain, we could only compare part of our results with the previous work 5. Although we partially use the same formulas as Yang et al.5to initially estimate five of the geometric parameters, our method for single chain show advantages due to added refinement based on optimization step. The optimization based refinements improve the quality of reconstruction. Our results demonstrate that we also find accurate and precise values for the dual system parameters including the ¿z and a . 12/2007: G. Allan Johnson, Ph.D., Gabriel Howles-Banerji, Kristin Frinkley, Kathy Nightingale, Ph.D., Mark Palmeri, M.D., Ph.D.: Active staining for in vivo MR imaging in the brain Developed, implemented, and tested the methods for opening the blood-brain barrier and active staining. Provided insights into ultrasonic mechanisms of method. We describe a method for active staining to enhance the MR signal in the brain of live animals, i.e., in vivo contrast-enhanced brain imaging. The active staining method can be used to provide substantial increase in spatial resolutionmore than 5X greater than what has been seen previously. The same method can be used with specific probes and experimental protocols to provide quantitative localized measure of neuronal activity in the live rodent: functional MR imaging of the mouse or rat brain, again at spatial resolution substantially greater than has been demonstrated previously. Finally, with suitable probes and protocols, the method promises to help define the density of specific molecular targets in the brain in combination with very high-resolution 3D in vivo anatomic definition.