MOLECULES BOUND TO AMYLOID FIBRILS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项研究将使用鳗鱼成像结合到阿尔茨海默病S相关的β-淀粉样纤维的碘化小分子。最近，几个碘标记的小分子已经通过一种专有的生化分析被鉴定出来，与淀粉样纤维具有高亲和力。为了评估这些化合物的放射性标记版本作为体内阿尔茨海默病S斑块造影剂的潜在用途，例如通过SPECT，本研究试图开发一种体外成像方法，以更快地评估这些分子的哪些非放射性标记版本具有以下特征：1)高亲和力，2)高密度，3)高选择性标记淀粉样纤维。如果成功，这项研究将导致一种非基于放射性的方法，从视觉上确认小分子对组织中淀粉样沉积的特异性标记能力，这将极大地加快发现和开发治疗阿尔茨海默病的新诊断试剂--S。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This research will use EELS to image iodinated small molecules bound to Alzheimer¿s related beta-amyloid fibrils. Several iodinated small molecules have recently been identified by a proprietary biochemical assay to bind with high affinity to amyloid fibrils. In order to evaluate the potential utility of radiolabeled versions of these compounds as in vivo contrast agents for Alzheimer¿s plaques by e.g., SPECT, this research seeks to develop an in vitro imaging method to more rapidly assess which non-radiolabeled versions of these molecules have the characteristics of: 1) high affinity, 2) high density, and 3) high selectivity for labeling amyloid fibrils. If successful, this research will result in a non radioactivity-based method to visually confirm the capability of small molecules to specifically label amyloid deposits in tissues, which should dramatically expedite the discovery and development of new diagnostic agents for Alzheimer¿s disease.