Transgenic and Mutant Animals

转基因和基因工程突变动物提供了最有价值的机制之一 建立体内模型以解开癌症的分子和遗传机制并进行评估 治疗和预防研究的新战略。高效的转基因动物的产生需要巨大的 只有在畜牧业和畜牧业、畜牧业和畜牧业方面有丰富经验才能获得的技术技能 外科手术、胚胎操作和显微操作。转基因动物共享服务使 转基因技术对威斯康星大学的成员来说是可获得和负担得起的。 综合癌症中心。它使用的是全校的人员、设施和设备 威斯康星大学生物技术中心的转基因动物设施。目前由提供的服务 转基因动物的核心包括： 原核显微注射法制备转基因小鼠和大鼠 小鼠和大鼠胚胎的冷冻保存 基因敲除小鼠的产生：靶向载体的产生，靶向胚胎干细胞的产生 细胞克隆和将ES细胞注射到宿主囊胚中产生嵌合体小鼠 菌株的再衍生与卵巢移植 其他与转基因和基因敲除小鼠相关的各种分子生物学和胚胎服务 生产 咨询和培训 此外，工作人员在使用服务过程中的任何时候都可以咨询。 花了相当多的时间讨论实验设计，并提供关于它们的过程的指导 他们自己也“正在或将会”实施。他们还培训调查人员超数排卵等技术， 交配，收集雌性小鼠生殖道用于胚胎冷冻，分离DMA用于 原核微注射、小鼠操作和繁殖方案，以及切断尾巴和基因组DNA 制备Southern blotting以鉴定正确突变的ES细胞。

Transgenic and genetically-engineered mutant animals provide one of the most valuable mechanisms for generating in vivo models for unraveling the molecular and genetic mechanisms of cancer and for evaluating new strategies for treatment and prevention studies. Efficient generation of transgenic animals requires great technical skill that is acquired only after extensive experience with animal husbandry and breeding, animal surgery, embryo manipulation and micromanipulation. The transgenic Animal Shared Service makes transgenic technology accessible and affordable to the members.of the University of Wisconsin Comprehensive Cancer Center. It uses the personnel, facilities and equipment of the campus-wide University of Wisconsin Biotechnology Center's Transgenic Animal Facility. Services currently provided by the Transgenic Animals Core include: Generation of transgenic mice and rats by pronuclear microinjection Cryopreservation of mouse and rat embryos ¿ Generation of knockout mice: generation of targeting vectors, generation of targeted embryonic stem (ES) cell clones, and generation of chimeric mice by injection of ES cells into host blastocyst Strain rederivations and ovarian transplants Other miscellaneous molecular biology and embryo services related to transgenic and knockout mouse production Consulting and training In addition, the staff are available for consulting at any point in the process of using the services. Considerable time is spent discussing experimental design and providing guidance on processes that they themselves are or will "be carrying out. They also train investigators in techniques such as superovulating, mating, and collecting reproductive tracts from female mice for embryo cryopreservation, isolating DMA for pronuclear micronjections, mouse handling and breeding protocols, and cutting tails and genomic DNA preparation Southern blotting to identify correctly mutated ES cells.