Core C: Biobank Core

胰腺癌研究的一个主要障碍是缺乏高质量的注释 PDAC各阶段的人体组织标本。这一困难是由于相对难以获得 胰腺及其突出的纤维成分，这使得在技术上难以获得 对于许多分析方法来说足够的流变细胞性。生物银行核心的总体目标是 成立的目的是协助PPG成员实验室积累和集中生物资源， 是胰腺癌研究所急需的具体目标是：（1）保持 集中的、分子注释的人源性鼠异种移植物核心，其可以富集和扩增 肿瘤细胞群，同时保持最接近的基因型和组织学特征， 亲本人类癌症; 2）提供分子注释的细胞系库。为了实现这些目标 我们将利用我们直接获得人类手术标本和小鼠肿瘤的优势， P01研究人员使用的生物材料已在 组织学和遗传学水平（与实验病理学核心合作）。这些材料 包括：由来自原发性和非原发性胰腺癌的人胰腺癌标本产生的连续传代的异种移植物， 转移性癌症;来自我们的具有不同肿瘤抑制基因突变的小鼠模型的PDAC细胞系; 和具有各种突变谱的原代小鼠胰腺导管细胞。这些资源应 提供标准化的蜂窝系统以促进实验研究。核心还保持着一个 几乎所有可用的PDAC细胞系的储存库;这些细胞系已经过高分辨率CGH检测， 表达谱，使研究人员能够检索所需的细胞系与适当的基因组图谱。 核心将与P01的项目密切合作，提供生物材料和援助， 使用异种移植物和原代细胞的实验的设计和解释。内核将寻求 确保高水平的标本质量控制和标准化的协议，以确保有效的 P01项目之间的结果转换。

A major obstacle to research in pancreatic cancer has been lack of access to high-quality annotated specimens of human tissues from all stages of PDAC. This difficulty is due to the relative inaccessibility of the pancreas and its prominent fibrous component, which make it technically difficult to obtain samples of sufficient heoplastic cellularity for many analytic approaches. The overall goal of the BioBank Core will be established to assist PPG member laboratories in the accrual and centralization of biologic resources that are critically required for pancreatic cancer research. Specific aims are directed at: 1) maintaining a centralized, molecularly annotated human-derived murine xenograft core which'can enrich and amplify the neoplastic cell population while maintaining the genotypic and histologic character closest to that of the parent human cancer; 2) providing a molecularly annotated cell-line repository. To accomplish these aims we will take advantage of our immediate access to human surgical specimens and mouse tumors to provide P01 investigators with biological materials that have been subjected to extensive characterization at the histologic and genetic levels (in collaboration with the Experimental Pathology Core). These materials include: serially passaged xenografts created from human pancreatic cancer specimens from primary and metastatic cancers; PDAC cell lines from our mouse models with varying tumor suppressor gene mutations; and primary mouse pancreatic ductal cells with various mutational profiles. Together, these resources should provide standardized cellular systems to facilitate experimental studies. The Core also maintains a repository of nearly all available PDAC cell lines; these have been subjected to high-resolution CGH and expression profiling, enabling investigators to retrieve needed cell lines with an appropriate genomic profile. The Core will interact closely with the Projects of the P01 to provide the biological materials and aid in the design and interpretation of experiments employing the xenografts and primary cells. The Core will seek to ensure a high level of quality control of specimens and standardization of protocols to ensure the effective translation of results between P01 Projects.