INVESTIGATING THE FUNCTION OF NS4B OF BVDV

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 牛病毒性腹泻病毒，类似于HCV，是黄病毒科的成员，并且在该类别中，它是瘟病毒。Matt Paulson已在BVDV中鉴定出HCV NS 4 B同源物的突变位点，该突变位点导致感染后耐药性增加（感染后细胞未死亡）。我们的目标是首先确定正常和突变蛋白质的细胞相互作用伙伴。我们已经比较了几种分离方法，并使用磁珠到目前为止，我们已经确定了一个预期的相互作用的合作伙伴。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Bovine Viral Diarrhea Virus, similarly to HCV, is a member of Flaviviridae, and within this category, it is a Pestivirus. Matt Paulson has identified a mutation site of the HCV NS4B homologue in BVDV which resulted in an increased resistance following the infection (the cells did not die after infection). We aim to first identify the cellular interacting partners of the normal and the mutant protein. We have compared several isolation methods and using magnetic beads so far we have identified one of the expected interacting partners.