CHEMICAL MECHANISMS OF ENZYMES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 由酶进行的反应在相对较低的温度下进行，通常是在水环境中近中性的pH下进行，通常产率和ee都是100%。酶几乎不会产生不需要的副产物，这可以归因于对所需反应的惊人控制水平。这一研究领域提出关于酶和代谢途径的定量问题，希望了解酶如何执行困难的化学转化和对恶劣环境的反应。使用过渡金属作为辅因子的酶特别令人感兴趣。虽然这些项目侧重于细胞中的酶机制和调节，但实验方法来自化学和分子生物学的不同领域。这些项目为协作工作提供了许多机会。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Reactions carried out by enzymes occur at comparatively low temperatures, usually at near-neutral pH in an aqueous environment, typically with 100% yields and 100% ee's. Enzymes almost never make undesired side products, which can be attributed to an amazing level of control over the desired reaction. This area of research asks quantitative questions about enzymes and metabolic pathways, in hopes of learning how enzymes perform difficult chemical transformations and respond to hostile environments. Enzymes that use transition metals as cofactors are of special interest. While the projects focus on enzyme mechanism and regulation in cells, experimental approaches are drawn from diverse areas of chemistry and molecular biology. The projects afford many opportunities for collaborative work.