SMOOTH MUSCLE CELL ARACHIDONIC ACID RELEASE, MIGRATION, AND PROLIFERATION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 药理学证据表明，由一种或多种Ca 2+非依赖性磷脂酶A2（iPLA 2）产生的脂质产物通过平滑肌细胞（SMC）Ca 2+信号传导和花生四烯酸的释放参与血管张力的调节。然而，最近鉴定出的iPLA 2家族的新成员（每个都可被（E）-6-（溴亚甲基）-3-（1-萘亚甲基）-2H-四氢吡喃-2-酮取代）使得确定性鉴定介导这些过程的特异性酶变得困难.因此，我们使用iPLA 2b-/-小鼠来证明iPLA 2b负责大部分毒胡萝卜素和离子载体（A23187）诱导的花生四烯酸从SMC释放。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Pharmacologic evidence suggests that the lipid products generated by one or more Ca2+-independent phospholipases A2 (iPLA2s) participate in the regulation of vascular tone through smooth muscle cell (SMC) Ca2+ signaling and the release of arachidonic acid. However, the recent identification of new members of the iPLA2 family, each inhibitable by (E)-6-(bromomethyl ene)-3-(1-naphthal enyl )- 2 H- t e t r a hy dr o py r a n- 2 - one, has rendered definitive identification of the specific enzyme(s) mediating these processes difficult. Accordingly, we used iPLA2b-/- mice to demonstrate that iPLA2b is responsible for the majority of thapsigargin and ionophore (A23187)-induced arachidonic acid release from SMCs.