Wiring of Gene Expression in the Early Meiotic Program

微阵列核心设施将提供微阵列服务和生物信息学服务， 方案项目。微阵列服务将包括自制的基因表达微阵列 （尽管我们正在逐步淘汰）;目录和定制Agilent微阵列;以及目录Affymetric微阵列，用于基因表达和ChIP-芯片分析。通常，微阵列核心设施将从研究者处接收冷冻细胞沉淀。冷冻沉淀将被处理以产生RNA，并且该RNA将被标记并与微阵列杂交。数据将被处理并加载到SQL数据库。研究者将在数据库中访问这些部分处理的数据。因此，核心设施可以处理从冷冻细胞团到部分处理数据存储的所有步骤。该计划项目的所有成员将大量使用这一设施，方法和分析的通用性，以及所有相关数据在同一数据库中的存在，将大大提高我们解释数据的能力。

The Microarray Core Facility will provide microarray services, and bioinformatic services, to the members of the Program Project. The microarray services will include home-made gene expression microarrays (although we are phasing this out); catalogue and custom Agilent microarrays; and catalogue Affymetric microarrays, for both gene expression and ChlP-Chip analysis. Typically the Microarray Core Facility will receive a pellet of frozen cells from the investigator. The frozen pellet will be processed to yield RNA, and this RNA will be labeled and hybridized to a micorarray. Data will be processed and loaded to an SQL database. Investigators will access this partially processed data in the database. Thus the Core facility can handle all steps from the frozen cell pellet to the storage of partially processed data. All members of the Program Project will use this facility heavily, and the commonality of methods and analysis, and the presence of all relevant data in the same database, will greatly increase our ability to interpret the data.