Development of Streamed Ion Deposition for Efficient Plasmid DNA Delivery

描述（由申请人提供）：本申请提出探索用于皮肤的新型DNA递送平台，其利用在等离子体中产生的离子流（带电气流）来促进DNA的摄取。这种新的物理DNA递送技术与其他技术的不同之处在于它不使用化学或生物制剂。它也不同于其他物理DNA递送方法，因为它不需要任何设备和皮肤之间的接触;因此，它是非侵入性的。初步研究表明，有可能在体外将外源分子递送到细胞，并在体内将质粒DNA递送到正常小鼠皮肤，而不会出现使用其他电方法时所注意到的不利刺激。基于这些发现，该提议的假设是流离子沉积可用于有效地将DNA递送到皮肤。将通过执行表征其递送编码报告分子的质粒的能力的特定目标来测试该假设。还将针对递送编码抗原和分泌蛋白的质粒进行测试，以探索该方法用于预防和治疗用途的能力。 公共卫生相关性：这项研究与公共卫生有关，因为其结果可能导致治疗许多类型的疾病，这些疾病需要将治疗性蛋白质分泌到血液中。此外，它可能会导致一种更好的方法来应用DNA疫苗对抗传染病。

DESCRIPTION (provided by applicant): This application proposes to explore a novel DNA delivery platform for skin that utilizes a stream of ions generated in plasma (charged gas stream) to facilitate the uptake of DNA. This new physical DNA delivery technology differs from other technologies in that it does not utilize chemical or biological agents. It also differs from other physical DNA delivery methods in that it does not require contact between any devices and the skin; therefore, it is noninvasive. Preliminary studies have shown that it is possible to deliver foreign molecules to cells in vitro and plasmid DNA to normal mouse skin in vivo without the adverse stimulation noted when other electrical methodologies are used. Based on these findings, the hypothesis of this proposal is that streamed ion deposition can be used for efficient DNA delivery to skin. This hypothesis will be tested by performing specific aims that characterize its capacity to deliver plasmids encoding reporter molecules. It will also be tested with respect to delivering plasmid encoding an antigen and a secreted protein to explore the methods capacity for prophylactic and therapeutic use. PUBLIC HEALTH RELEVANCE: This study is relevant to public health as the results may lead to treatments for many types of diseases that require the secretion of a therapeutic protein into the blood. In addition, it may lead to a better means of applying DNA vaccines against infectious diseases.