CORE C MOUSE TRANSGENIC AND MAGNETIC RESONANCE IMAGING CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该中心的目标是提供设施和技术专长，支持涉及老鼠模型的项目，以此作为解决重要的生物和生物医学问题的手段。CORE的服务包括通过原核注射产生转基因小鼠，胚胎干细胞的基因打靶，胚胎干细胞注入囊胚，再衍生，小鼠胚胎冷冻保存，以及共享小鼠品系的进口、维护、分布和小动物磁共振成像。今年的新组件包括扩展的体内成像能力，具有高分辨率超声波和微型CT组件。小鼠转基因组件/微CT由Lucy Liu博士负责监督，并雇用了两名全职研究助理。磁共振成像和超声部件由Ilka Pinz博士指导，并聘请了一名全职研究助理。这一核心设施为我们研究所内的所有研究人员提供了服务，并将转基因和成像服务扩展到科布尔州的研究人员和新英格兰地区的其他研究人员。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objectives of this Core are to provide facilities and technical expertise in support of projects involving mouse models as a means of addressing important biological and biomedical questions. Services of the core include generation of transgenic mice by pronuclear injection, gene targeting in embryonic stem (ES) cells, ES cell injection into blastocysts, re-derivation, mouse embryo cryopreservation, and the importation, maintenance, distribution of shared mouse strains, and small animal magnetic resonance imaging. New components for this year include expanded in vivo imaging capacity with a high resolution ultrasound and a microCT component. The Mouse Transgenic components/microCT are overseen by Lucy Liaw, Ph.D., and employs two full-time research associates. The magnetic resonance imaging and the ultrasound components are directed by Ilka Pinz, Ph.D., and employ one full-time research assistant. This core facility has served all investigators within our Institute, and has extended transgenic and imaging services to investigators in COBRE states and other investigators in the New England region.