CORE--BIOMATERIALS

PKD生物材料研究中心的总体目标是建立和维护一个人类和动物生物材料的储存库，供堪萨斯州PKD中心的研究人员使用。该核心将为开发高质量的研究材料提供资源和专业知识，包括ADPKD、ARPKD和正常人类肾脏组织的上皮细胞原代培养、组织标本、DNA、RNA和细胞蛋白。该核心还将建立和维护来自具有独特PKD基因突变的小鼠的永生化细胞系。核心的优势在于，它将提供一个中心实验室，其专门的研究人员在1)采购人体组织并接受适当处理人体生物样本方面的培训，2)无菌收集囊性和非囊性组织和囊液的方法， 3)组织学准备4)蛋白质、RNA和DNA的提取，5)畜牧业、计时育种和动物基因分型，6)从特定细胞类型制备用于PKD小鼠的细胞系。该核心的具体目标是：目标1：建立和维护一个人类PKD生物材料储存库。目的2：从具有导致PKD的独特基因缺陷的动物身上产生和维持条件永生化的肾上皮细胞。目的3：为研究PKD细胞表型的体外方法提供技术支持和培训。Core将向堪萨斯PKD中心的成员以及堪萨斯大学医学中心和其他大学的合作者提供独特的PKD生物材料。

The overall goal of the PKD Biomaterials Research Core is to establish and maintain a repository for human and animal biological materials for.investigators within the Kansas PKD Center. The core will provide resources and expertise for the development of high quality research materials, including primary cultures of epithelial cells, histological specimens, DNA, RNA and cell proteins from ADPKD, ARPKD and normal human kidney tissues. The core will also establish and maintain immortalized cell lines from mice with unique PKD gene mutations. The strength of the core is that it will provide a central laboratory with dedicated research staff experienced in 1) the procurement the human tissues and trained on the proper handling of the human biological samples, 2) methods of aseptic collection of cystic and non-cystic tissue and cyst fluid, 3) histological preparation 4) protein, RNA and DNA extraction, 5) animal husbandry, timed breeding and animal genotyping, and 6) preparation of cell lines from specific cell types for PKD mice. The specific aims of the Core are: Aim 1: Establish and maintain a human PKD biomaterial repository. Aim 2: Generate and maintain conditionally immortalized renal epithelial cell cultures from animals with unique gene defects that cause PKD. Aim 3: Provide technical support and training for in vitro methods for the investigation of the PKD cellular phenotype. The Core will provide unique PKD biomaterials to members of the Kansas PKD Center and to collaborators at the Kansas University Medical Center and other universities.