权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Biomedical Research Core 1 - Biomarkers, Biomaterials, and Cellular Models Core

生物医学研究核心 1 - 生物标志物、生物材料和细胞模型核心

基本信息

批准号：
10214614
负责人：
DARREN P. WALLACE
金额：
$ 19.54万
依托单位：
UNIVERSITY OF KANSAS MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-15 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10214614
关键词：
3-Dimensional Age Alcohol consumption Animal Model Autosomal Dominant Polycystic Kidney Beta-N-Acetylglucosaminidase Biocompatible Materials Biological Assay Biological Markers Biomaterials Research Biomedical Research Blood Blood Urea Nitrogen CCL2 gene Caffeine Cell Line Cell Proliferation Cell model Cells Chronic Kidney Failure Clinic Clinical Clinical Research Cohort Studies Collagen Collection Creatinine Cultured Cells Cyst Cyst Fluid Cystic Fibrosis Transmembrane Conductance Regulator DNA Databases Development Diagnosis Disease Duct (organ) structure Early Intervention Epithelial Cell Proliferation Epithelial Cells Epithelial cyst Family Fluids and Secretions Formalin Freezing Gene Mutation Gene Targeting Generations Genes Goals Gold Growth Hospitals Human Immunoassay In Vitro Individual Industry Injury to Kidney Investigation Kansas Kidney Knock-out LCN2 gene Laboratories MRI Scans Measurement Medical History Molecular Monitor Mutation Nephrons Paraffin Embedding Participant Pathogenicity Pathway interactions Patients Pharmaceutical Preparations Plasma Preparation Provider Race Reagent Recording of previous events Research Research Personnel Risk Risk Factors Sampling Serum Services Siblings Site Smoking History Testing Therapeutic Therapeutic Agents Therapeutic Intervention Time Tissue Embedding Tissues Training Translating Translational Research Translations Universities Urine biomarker discovery biomarker panel cell immortalization cohort comorbidity detection platform drug testing effective therapy electrical measurement exosome experimental analysis high resolution imaging human tissue in vitro Assay in vitro Model interstitial cell kidney cell monolayer mutant novel novel therapeutics post gamma-globulins potential biomarker predictive marker rat KIM-1 protein recruit renal damage repository response sex specific biomarkers therapeutic evaluation urinary volunteer

项目摘要

Project Summary – Abstract – BRC1 The Biomarkers, Biomaterials, and Cellular Models Core is comprised of four laboratories that provide valuable human biospecimens and core services to internal and external Core users. The PKD Biomarkers Laboratory maintains a repository of serum, plasma, urine, and urinary exosomes from individuals with early- stage ADPKD that are being monitored in the Early PKD Observational Cohort (EPOC), a longitudinal clinical study. The repository also banks samples from at-risk siblings of these ADPKD participants, normal volunteers, and patients with established ADPKD that come to the PKD clinic at the University of Kansas Hospital. The current lack of sensitive and specific biomarkers for ADPKD is a major impediment in the development of new therapies. The Core will assist investigators in the discovery of effective biomarkers for diagnosing and monitoring the progression of early-stage ADPKD, a critical time before there is reversible damage to the kidneys. The Core will also perform in-house biomarker analysis of clinic samples to assist PKD investigators on the evaluation of therapeutic interventions. The PKD Biomaterials Laboratory maintains an established repository of a broad spectrum of human ADPKD biospecimens, including fixed and frozen cystic tissues, cyst fluids, and primary cultures of cyst epithelial cells. The Core has been critical for providing ADPKD biospecimens to academic and industry investigators for the past 14 years. The PKD Cellular Models Laboratory assists investigators in the use of human primary ADPKD cells, normal human kidney (NHK) cells and immortalized renal cell lines in carefully controlled in vitro assays. The Core has considerable expertise with in vitro models for the investigation of pathways involved in cyst epithelial cell proliferation, CFTR-dependent Cl- and fluid secretion, and in vitro cyst formation. The Core will continue to provide service and training to investigators on PKD cellular models to investigate mechanisms for cyst growth and for evaluating potential therapeutic compounds. The PKD Gene Targeting Laboratory assists in the generation of novel PKD reagents through gene- editing and assists Core users in gene-targeting approaches to test specific hypotheses. The Core will introduce specific mutations in PKD1 or PKD2 in immortalized cells from human collecting ducts, a prominent site for cyst formation in ADPKD. This enables investigators to examine the cellular response to a PKD mutation using isogenic normal and PKD mutant cell lines. The overall goal of the PKD Biomarkers, Biomaterials, and Cellular Models Core is to provide human ADPKD biospecimens and cellular models to assist investigators in translational research.

项目摘要-摘要- BRC1