CORE--Assay

由于肽激素和循环底物的测量将提供关键的实验 端点的每一个单独的项目，构成这个计划项目，资金要求为一个 检测核心实验室。该实验室的主要目的是建立和执行以下检测： 完成这三个项目所必需的关键调节肽的测量。 核心的常规测定包括胰岛素、瘦素、葡萄糖、皮质酮、胃泌素释放肽， 和胆囊收缩素。这一核心还将负责调整商业上可用的成套工具 测量其他激素和肽所必需的，标记用作示踪剂的肽，处理组织 用于肽的测定以及新技术和测定的开发。这个核心将被利用 广泛应用于所有3个项目。

Since the measurement of peptide hormones and circulating substrates will provide key experimental endpoints for each of the separate projects that make up this program project, funding is requested for an Assay Core laboratory. The primary purpose of this laboratory will be to establish and perform assays for the measurement of the key regulatory peptides necessary for the completion of the three projects. Routine assays for the core will include insulin, leptin, glucose, corticosterone, gastrin releasing peptide, and cholecystokinin. This core will also be responsible for adapting commercially available kits necessary to measure other hormones and peptides, labeling peptides for use as tracers, processing tissues for assay of peptides and development of new techniques and assays. This core will be utilized extensively by all 3 of the individual projects.