Electron Microscopy Core

该计划项目的EM核心有两个相关的目的-支持仪器必不可少的 本补助金的四个项目中提出的研究，并促进正在开发的方法的应用 在这些项目中，合作和独立的努力。核心的主要成分是电子 在哈佛医学院（HMS）和布兰迪斯大学的显微镜设施。HMS设施将 有四台FBI显微镜：Tecnai F20，Tecnai T12，CM 10和Polara F30。F30、F20和 T12显微镜配备了低温载物台; F30和F20配备了场发射枪。布兰迪斯 该设施还拥有四台FBI仪器：一台Morgagni M268和EM 20，一台CM 12和一台Tecnai F30。最后一 三个有低温阶段; F30有一个场发射枪和一个能量过滤器。核心支持的操作 这些显微镜和每个地点的设施经理的工资，以及必要的辅助设备， 设备.核心也有一个计算组件。它可以升级所有标准的电磁软件， 包括程序套件，如FREALIGN，IMAGIC和SPIDER，通过结构生物学 计算网格（SBGrid）由HMS分子和细胞动力学中心建立。最后， 通过提供EM和计算设施，核心允许P.I.的这些项目进行合作 此外，还与各机构的其他小组合作，对博士后研究员和学生进行有关方法的培训。 相关性：电子显微镜是连接低分辨率和高分辨率的关键实验工具。 结构方法大型组装将成为未来人类疾病干预的目标， 显微镜将是这些复合物的结构和功能的核心

The EM core of this Program Project has two related purposes - to support instrumentation essential for the research proposed in the four projects of this grant and to facilitate application of the methods being developed in these projects to collaborative and independent efforts. The principal components of the core are the electron microscopy facilities at Harvard Medical School (HMS) and at Brandeis University. The HMS facility will house four FBI microscopes: a Tecnai F20, a Tecnai T12, a CM10, and a Polara F30. The F30, the F20, and the T12 microscopes are equipped with cryo-stages; the F30 and the F20 have field-emission guns. The Brandeis facility also houses four FBI instruments: a Morgagni M268, and EM20, a CM12, and a Tecnai F30. The last three have cryo-stages; the F30 has a field-emission gun and an energy filter. The core supports the operation of these microscopes and the salary of a facilities manager at each location, as well as necessary ancillary equipment. The core also has a computational component. It maintainsand upgrades all standard EM software, including program suites such as FREALIGN, IMAGIC, and SPIDER, through a structural biology computational grid (SBGrid) established by the Center for Molecular and Cellular Dynamics at HMS. Finally, by providing EM and computational facilities, the core allows the P.I.'s of these projects to collaborate with other groups at various institutionsand to train postdoctoral fellows and students in the relevant methods. Relevance: Electron microscopy is the key experimental tool that bridges between low- and high-resolution structural methods. Large assemblies will be targets of future interventions in human disease, and electron microscopy will be central for relating structure and function of these complexes