Role of Mitochondrial Aldehyde Dehydrogenases in Ethanol Metabolism and Toxicity

描述(申请人提供)：长期酗酒有多种病理后果，包括对肝脏、大脑和其他器官的损害。这些病理效应中的一些可以归因于酒精代谢。乙醇被乙醇脱氢酶(ADHS)、乙醇诱导的细胞色素P450(CYP2E1)和过氧化氢酶代谢为乙醛。乙醇的许多毒性作用是由于乙醛及其对分子靶标的作用。蛋白质、脂质和线粒体DNA。因此，乙醛代谢的作用对于了解饮酒所致组织损伤的发病机制可能是至关重要的。乙醛代谢受损与人类乙醛脱氢酶(ALDH)酶的多态性有关；这些酶可能影响饮酒行为和发展为酒精性肝病的风险。已知线粒体ALDH2和细胞质ALDH1A1酶在乙醛解毒中起主要作用。人类ALDH2具有2个不同的等位基因，即ALDH2*1和ALDH2*2。ALDH2*2纯合子个体完全缺乏ALDH2活性，而ALDH2*1/2*2杂合子个体仅表现出正常ALDH活性的30-50%。这种酶活性的改变有生理上的影响。例如，在饮用等量酒精后，ALDH2*2纯合子个体的血液乙醛水平是几乎检测不到乙醛的ALDH2*1/2*1个体的6-20倍。ALDH1B1是一种线粒体酶，与ALDH2有75%的同源性，也可能参与乙醛的代谢。虽然关于ALDH2和ALDH1A1的信息很多，但对ALDH1B1的了解很少。我们对R21拨款申请的工作假设是双重的。首先，我们假设线粒体ALDH1B1是一种功能酶，通过代谢乙醛和脂质过氧化相关的醛来保护细胞。其次，我们假设ALDH2*2蛋白可能通过与其他ALDH相互作用而使其失活。在这项应用的两年期间，我们建议从以下几个方面对人和小鼠ALDH1B1蛋白进行表征：(A)酶功能(乙醛和脂质过氧化醛的代谢)，(B)在小鼠和人类组织中的表达模式，以及(C)其对醛诱导的毒性和细胞凋亡的保护作用。这些将使用重组ALDH1B1蛋白和表达、过度表达或缺乏该蛋白的细胞系进行研究。此外，我们将确定ALDH2*2蛋白是否同时失活ALDH2*1酶和ALDH1B1。这将通过研究使用重组蛋白质和表达这些蛋白质的细胞系形成异四聚体来实现。

DESCRIPTION (provided by applicant): Chronic alcohol abuse has a variety of pathological consequences including damage to liver, brain and other organs. Some of these pathological effects can be attributed to ethanol metabolism. Ethanol is metabolized to acetaldehyde by the alcohol dehydrogenase enzymes (ADHs), ethanol-inducible cytochrome P450 (CYP2E1) and catalase. Many of the toxic effects of ethanol are due to acetaldehyde and its actions on molecular targets viz. proteins, lipids and mitochondrial DNA. As a result, the role of acetaldehyde metabolism may be crucial for understanding the pathogenesis of tissue injury caused by alcohol consumption. Impaired acetaldehyde metabolism is associated with polymorphisms in the human aldehyde dehydrogenase (ALDH) enzymes; these may influence drinking behavior and risk for developing alcoholic liver disease. Mitochondrial ALDH2 and, to a lesser extent, cytosolic ALDH1A1 enzymes are known to play a major role in acetaldehyde detoxification. Human ALDH2 is polymorphic with 2 distinct alleles, ALDH2*1 and ALDH2*2. ALDH2*2 homozygous individuals are completely devoid of ALDH2 activity, whereas ALDH2*1/2*2 heterozygous individuals exhibit only 30-50% of the normal ALDH activity. This altered enzyme activity has physiological ramifications. For example, after equivalent amounts of alcohol are consumed, blood acetaldehyde levels in ALDH2*2 homozygous individuals are 6-20 times higher than those in ALDH2*1/2*1 individuals in whom acetaldehyde is hardly detectable. ALDH1B1 is a mitochondrial enzyme that is 75% identical to ALDH2 and also may be involved in acetaldehyde metabolism. Although there is a considerable amount of information about ALDH2 and ALDH1A1, very little is known about ALDH1B1. Our working hypothesis for this R21 grant application is two-fold. First, we hypothesize that the mitochondrial ALDH1B1 is a functional enzyme that protects cells by metabolizing acetaldehyde and lipid peroxidation-associated aldehydes. Second, we hypothesize that ALDH2*2 protein may inactivate other ALDHs by interacting with them. For the 2-year period of this application, we propose to characterize the human and mouse ALDH1B1 proteins in relation to: (a) enzymatic function (metabolism of acetaldehyde and lipid peroxidation aldehydes), (b) expression pattern in both mouse and human tissues, and (c) its protective role against aldehyde-induced toxicity and apoptosis. These will be studied using recombinant ALDH1B1 proteins and cell lines expressing, over-expressing or lacking this protein. In addition, we will determine whether ALDH2*2 protein inactivates both ALDH2*1 enzymes and ALDH1B1. This will be accomplished by studying the formation of heterotetramers using recombinant proteins and cell lines expressing these proteins.