Ecology and Genetics of An. gambiae on Islands

转基因蚊子（GMM）品系的开发进展将用于疟疾蚊子媒介的遗传控制计划，已经达到了一个点，参与这项研究的大多数科学界开始考虑现场试验。以下建议是由一组参加题为“人类传染病的基因工程节肢动物载体”会议的载体生物学专家提出的：“在阻断载体中寄生虫发育的分子策略方面取得的令人兴奋的进展远远超过了在任何转基因释放田间试验之前必须进行的有关田间地点和载体种群的基本工作。“帝国 学院，伦敦，2001年9月14日至15日。 我们认为，GMM田间试验最有希望的地点将是撒哈拉以南非洲沿海的岛屿。在这个探索中！发展研究补助金建议（R21），我们寻求支持，以开展旨在评估两个岛屿的适合性的研究。我们选择了非洲东海岸的一个岛屿和西部的一个岛屿来探索这项赠款申请。基于对现有数据的批判性分析，我们选择研究奥兰戈岛上的冈比亚按蚊种群，这是西非比贾戈群岛和东非大科摩罗群岛的一部分。我们的初步评估将包括： (1)收集了安的基本生态和生物数据。每个地点的冈比亚，包括相对种群密度和成虫分布; (2)评估岛屿人口在遗传上与最近的大陆人口和邻近岛屿人口隔离的程度，并说明两个目标岛屿中每一个岛屿人口的遗传结构; (3)建立一个地理信息系统，描述每个地点的生态，并与生态学和种群遗传学研究的信息充分结合。

Progress toward the development of genetically modified mosquito (GMM) strains to be used in a genetic control program for mosquito vectors of malaria has reached a point where most of the scientific community involved in this research are beginning to think about field trials. The following recommendation was made by a group of experts in the field of vector biology participating in a meeting entitled "Genetically Engineered Arthropod Vectors of Human Infectious Diseases": "The exciting progress in molecular strategies for blocking parasite development in vectors has far outpaced the related and essential work on field sites and vector populations that must precede any GM release field trial." Imperial College, London 14-15 September, 2001. We believe that the most promising sites for GMM field trial sites would be on islands off the coast of sub-Saharan Africa. With this Exploratory! Developmental Research Grant proposal (R21) we seek support to initiate studies aimed at assessing the suitability of two island sites. We have selected one island off the east coast of Africa and one off the west to explore for this grant application. Based on a critical analysis of available data we chose to study Anopheles gambiae populations on Isla de Orango, part of the Bijago Archipelago in West Africa and Grande Comore in East Africa. Our initial assessment will include: (1) collection of basic ecological and biological data for An. gambiae at each site, including relative population densities and adult distribution; (2) an assessment of the degree to which island populations are genetically isolated from the nearest mainland populations and from populations on neighboring islands, and a description of the genetic structure of populations on each of the two target islands; (3) creation of a GIS system that describes the ecology of each site and is fully integrated with information from the ecology and population genetics studies.