TMEM103 in Eosinophil Development

描述(申请人提供)：嗜酸性粒细胞与许多过敏性疾病有关，包括哮喘、特应性皮炎、药物反应和慢性荨麻疹。无数与嗜酸性粒细胞相关的疾病使我们研究了从造血干细胞发展而来的嗜酸性粒细胞，试图了解促使骨髓中产生嗜酸性粒细胞的信号。发育中的嗜酸性粒细胞的基因图谱表明，一种新的假想蛋白质以mRNA的形式表达。TMEM103在IL-5刺激的CD34+细胞、骨髓和成熟的嗜酸性粒细胞中表达。该转录本TMEM103已被证明与核糖体相关，该蛋白已通过蛋白质印迹分析在发育中的嗜酸性粒细胞中得到鉴定。这项拨款建议通过提高TMEM103蛋白质的克隆抗体，然后对蛋白质进行纯化和测序来进一步验证TMEM103的存在。此外，TMEM103的功能将通过RNA沉默来研究，随后将研究形态和转录图谱的变化。公共卫生相关性：嗜酸性粒细胞是与哮喘和过敏性疾病有关的白细胞。这个项目探索了TMEM103，一种新的蛋白质，在嗜酸性粒细胞中的作用。

DESCRIPTION (provided by applicant): Eosinophils are associated with many allergic diseases including asthma, atopic dermatitis, drug reactions and chronic urticaria. The myriad of eosinophil related diseases has led us to investigate the development of eosinophils from hematopoietic stem cells in an attempt to understand the signals prompting eosinophil production in the bone marrow. Gene profiling of developing eosinophils has shown that a novel hypothetical protein is expressed as mRNA. TMEM103 mRNA is expressed in IL-5 stimulated CD34+ cells, bone marrow and in mature eosinophils. The transcript, TMEM103, has been shown to be associated with ribosomes and the protein has been identified by western blot analysis in developing eosinophils. This grant proposes to further verify the existence of TMEM103 by raising monoclonal antibodies to the protein, then purifying and sequencing the protein. Further, the function of TMEM103 will be investigated by RNA silencing followed by studies investigating morphological and transcription profile changes. PUBLIC HEALTH RELEVANCE: Eosinophils are leukocytes associated with asthma and allergic diseases. This project explores the role of TMEM103, a novel protein, in eosinophils.