UNDERSTANDING MECHANISMS OF hESC SELF-RENEWAL AND CELL FATE COMMITMENT

该计划的首要主题将是定义 hESC的基本自我更新机制以及它们如何被指定为不同的谱系。每个 的研究项目是互补的，但高度协同作用，并建立在一个共同的生物 平台该计划强调使用最新的hESC培养技术，并将 与东南部科学界保持高度互动。这三个项目将： 和早期细胞命运承诺的细胞方面; -确定基因组在 - 并应用现有技术来表征hESC中的糖基化模式， 分化细胞这三个核心将：-作为该计划和东南部的资源， 维持和分配hESC，提供为期2周的hESC强化培训计划 - 开发新技术， 解决制约hESC领域进展的主要问题; -提供行政、后勤支持 为该计划，并作为与东南互动的渠道。该计划将大大 加速格鲁吉亚大学在hESC领域取得的科学进步， 东南部的机构。总的来说，这个团队的发现将对我们的研究产生重大影响。 了解基本的hESC生物学和人类发育。所有这些发现都将至关重要 用于开发将hESC用于细胞治疗的策略， 退行性疾病和慢性损伤的修复。

The overarching theme of this Program will be to define basic self-renewal mechanisms of hESCs and how they become specified into different lineages. Each of the research projects is complementary but highly synergistic and built on a common biological platform. The Program places a strong emphasis on using the latest hESC culture technology and will be highly interactive with the Southeast scientific community. The 3 Projects will: - address molecular and cellular aspects of early cell fate commitment; - determine how the genome is remodeled during differentiation;- and apply state of the art technology to characterize glycosylation patterns in hESC and differentiated cells. The 3 Cores will: - function as a resource for the Program and the Southeast by maintaining and distributing hESCs, providing an intensive 2-week Training Program in hESC technology and functioning as a general technical resource; -establish new technologies designed to solve major problems that restrict progress in the hESC area; - provide administrative, logistical support for the Program and act as a conduit for interactions with the Southeast. The Program will significantly accelerate scientific advances made in the hESC area at the University of Georgia and at partnering Institutions in the Southeast. Overall, discoveries made by this team will have a major impact on our understanding of basic hESC biology and human development. All of these discoveries will be critical for the development of strategies where hESCs are to be used in cell therapies for treating degenerative disease and repair of chronic injury.