ANATOMIC PATHOLOGY CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 解剖病理学核心由尸检、组织学和组织采集和分配单元组成，负责尸检、组织采集和分配、固定、处理、载玻片制备、常规和特殊染色。 中心的政策是，如果可能的话，确定所有在中心死亡的动物的死亡原因，无论是自然原因还是实验原因。 这使我们能够监测和记录殖民地内发生的所有传染因子和疾病过程，并提供了发现新疾病的机会，这可能是有用的模型。 此外，该中心的大多数主要研究领域在很大程度上依赖于解剖病理学支持作为研究的一个组成部分。 2008年，该中心共进行了781次尸检和378次活组织检查。解剖病理学核心实验室仪器包括3台Microm HM 325切片机和一台自动Leica RM 2145切片机，用于制备常规H&E和特殊染色的薄和厚组织切片;共聚焦显微镜切片，抗体和分子探针标记研究（在分子病理学核心中进行）。 我们还使用Leica ASP 300组织处理器、Tissue-Tek包埋站以及各种水浴、烤箱、显微镜和计算机工作站。 今年的核心制作了21，383张研究幻灯片和另外3，648个未切片的块。 按来源分类，核心科学家的总数为13，757张幻灯片和2，539个额外区块，附属科学家的总数为7，646张幻灯片和1，109个额外区块。我们打算在不久的将来实现载玻片染色和盖片过程的自动化，以提高制作的载玻片的一致性，并进一步减少我们的工作人员接触的化学品。 目前，我们雇用了三名全职组织学家，他们拥有40年的实验室经验。 艾滋病研究方案是核心服务的主要用户。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Anatomic Pathology Core consists of the Necropsy, Histology, and Tissue Collection and Distribution Units and is responsible for post-mortem examinations, tissue collection and distribution, fixation, processing, slide preparation, routine and special staining. It is a center policy that a cause of death is determined, if possible, for all animals that die at the center, whether of natural or experimental causes. This allows us to monitor and document all infectious agents and disease processes that occur within the colony and provides an opportunity to discover new diseases, which may be useful models. In addition, most major research areas at the center depend heavily on anatomic pathology support as an integral part of the research. In 2008, a total of 781 necropsies and 378 biopsies were performed by the Core. The Anatomic Pathology Core Laboratory instrumentation includes 3 Microm HM325 microtomes and one automated Leica RM2145 microtome for preparing thin and thick tissue sections for routine H&E and special staining; sections for confocal microscopy, labeling studies with antibodies and molecular probes (performed in the Molecular Pathology Core). We also utilize a Leica ASP 300 tissue processor, a Tissue-Tek embedding station, and assorted water baths, ovens, microscopes and a computer work station. This year the Core produced 21,383 research slides and an additional 3,648 un-sectioned blocks . These totals, when classified by source, are 13,757 slides and 2,539 additional blocks for core scientists and 7,646 slides and 1,109 additional blocks for affiliated scientists. We intend to automate the slide staining and cover-slipping processes in the near future to improve the consistency of the produced slides and to further reduce the chemical exposure to our staff. Currently we employ three full time histologists with a combined 40 years' laboratory experience. The AIDS research program is a major user of core services.