ANALYTIC CYTOLOGY CORE FACILITY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 德克萨斯大学埃尔帕索分校提议继续支持分析细胞学核心设施，这是一个多用户研究设施，致力于协助教师，工作人员和学生设计方案，使用Zeiss EM-10透射电子显微镜，Zeiss Axioskop荧光显微镜，图像分析和图像处理，以及最近获得的Leica Ultracut R超薄切片机。这使我们能够使用冷冻免疫标记技术。有16个教师的工作人员和学生有项目利用这个设施。我们预计，这一核心设施将是必不可少的新教师，我们已经提供了在病毒学和生理学的职位。本实验室提供的其他设备包括一台Pulnix 1001模拟/数字相机，带有一个闪光点捕获卡，使我们能够将数字图像发送到一台装有Image Pro软件的Dell Optiplex GX 110计算机。这些图像可以在柯达热升华打印机上打印。这为我们提供了一个数字暗室。Pelco固定微波减少了固定所需的时间。Sorvall临界点干燥装置用于另一个实验室中利用SEM的项目。Zeiss Axioskop配备了一台35 mm照相机、一台Optronics数码照相机、OPTIMAS软件、一台Gateway计算机、一台Hewlett Packard扫描仪、一台Nikon 35 mm扫描仪和一台Tektronix Phaser 740彩色激光打印机。还提供定制暗室设施。该设施将配备一名全职监督员/技术员，负责监督用户、监督和及时检查设备、制备化学溶液、修理超微切片机和暗室设备、维护设备日志、执行安全条例、处置废弃化学品、订购供应品和设备、监督新设备的安装以及维护库存。部分工资将提供给核心主任，以持续监督这些活动。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The University of Texas at El Paso proposes to continue support for the Analytical Cytology Core Facility, a multi-user research facility dedicated to assisting faculty, staff and students in the design of protocols, the use of the Zeiss EM-10 transmission electron microscope, the Zeiss Axioskop epifluorescence microscope, image analysis and image processing, as well as the recently acquired Leica Ultracut R ultramicrotome with a cryostage. This enables us to use cryoimmunolableling techniques. There are sixteen faculty whose staff and students have projects utilizing this facility. We anticipate that this core facility will be essential to new faculty to whom we have offered positions in virology and physiology. Additional equipment available in this laboratory includes a Pulnix 1001 analog/digital camera with a Flash point capture card which enables us to send digital images to a Dell Optiplex GX110 computer with Image Pro software. These images can be printed on a KODAK dye sublimation printer . This provides us with a digital darkroom. A Pelco fixation microwave reduces the amount of time needed for fixations. A Sorvall critical point drying apparatus is used for projects utilizing SEM in another laboratory. The Zeiss Axioskop is equipped with a 35 mm camera, an Optronics digital camera, OPTIMAS software, a Gateway computer, a Hewlett Packard scanner, a Nikon 35 mm scanner and a Tektronix Phaser 740 color laser printer. Custom darkroom facilities are also available. This facility will be staffed full-time by a supervisor/technician responsible for supervising users, oversight and timely inspection of equipment, preparing chemical solutions, repairing ultramicrotomes and darkroom equipment, maintaining log books for the equipment, enforcing safety regulations, disposing of waste chemicals, ordering supplies and equipment, supervising the installation of new equipment, and maintaining the inventory. Partial salary will be provided to the core director for ongoing oversight of these activiites.