ROTAVIRUS AND NORWALK VIRUS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 轮状病毒是婴幼儿胃肠炎的主要病原体。 它是一种二十面体病毒，直径为1000？，具有由三个同心衣壳层组成的复杂结构，其中包含11个dsRNA片段。几年来，我们一直试图确定这种医学上重要的病毒的各种形式的结构。 我们目前正在使用NCMI设施进行高分辨率的结构分析的转录能力，转录能力和转录活性形式的轮状病毒颗粒，了解分子机制的迷人的内源性转录过程中，这些病毒。 此外，我们还研究了轮状病毒的各种分子组成。 轮状病毒通过表达非结构蛋白NSP2和NSP5形成病毒囊。 这些病毒在病毒感染过程中形成的衣壳为基因组复制/包装和衣壳组装提供了排他性位点。 关于这些过程如何在这些病毒工厂内协调的结构基础知之甚少。 诺瓦克病毒是一种典型的诺如病毒，是急性流行性胃肠炎的病原。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rotavirus is a major pathogen of infantile gastroenteritis. It is an icosahedral virus, 1000 ¿ in diameter, with a complex architecture consisting of three concentric capsid layers enclosing 11 dsRNA segments. Over several years we have been trying to determine the structure of various forms of this medically important virus. We are presently using the NCMI facility to carry out high-resolution structural analysis of transcriptionally competent, transcriptionally incompetent and transcriptionally active forms of rotavirus particles, to understand molecular mechanisms underlying the fascinating process of endogenous transcription in these viruses. In addition, we also study various molecular components of rotavirus. Rotavirus viroplasms form by expression of nonstructural proteins NSP2 and NSP5. These viroplasms formed during viral infection provide excusive sites for genome replication/packaging and capsid assembly. Little is known about the structural basis of how these processes are coordinated within these viral factories. Norwalk virus is a prototype norovirus, which are causative agents of acute epidemic gastroenteritis.