CATION-PROTON ANTIPORTERS (CPAS): A NEW GROUP OF PH REGULATING TRANSPORTERS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 阳离子质子逆向转运体(CPA)是最近克隆并定位于蚊子消化道特定区域的一类新的pH调节转运蛋白。目前还没有对这些蚊子转运蛋白进行功能鉴定，它们的功能只是从序列同源性推断出来的，2H+被认为是进入细胞以换取1 Na+的外流。其中一个克隆序列AgNHA1已被建立在非洲爪哇卵母细胞异源表达系统中进行表达。当离子流出卵母细胞时，很难准确识别哪些阳离子通过质膜流出。目前的假设是，它可能是Na+或K+。我们已经对Na+进行了初步的通量测量，以支持拨款申请。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cation-Proton Antiporters (CPAs) are a new group of pH regulating transporter that have recently been cloned and localized to specific regions of the mosquito alimentary canal. No functional characterization has been performed for these mosquito transporters and their function has only been inferred from sequence homology, 2H+ are thought to enter the cell in exchange for efflux of 1 Na+. One of the cloned sequences, AgNHA1, has been setup to be expressed in the Xenopus oocyte heterologous expression system. As the ions are exiting the oocytes it is very difficult to identify exactly which cations are passing out through the plasma membrane. The current hypothesis is that it could be either Na+ or K+. We have performed preliminary flux measurements for Na+ in support of a grant submission.