ENGINEERING OF BICELLES FOR PULSED DIPOLAR ESR STUDY ON MEMBRANE PROTEINS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Bicelle提供了一种环境，在这种环境中，蛋白质可以与由具有生物活性的脂类组成的双层结构相互作用，这种双层结构被包装成足够小的颗粒，以便在溶液中快速翻滚，用于核磁共振，并在用于脉冲偶极ESR波谱的冷冻样品中实现高蛋白质浓度和均匀分布。因此，有必要设计合适的双分子结构并探索其在蛋白质-膜相互作用研究中的应用。我们追求的具体目标如下：(1)基于不同的长链和短链脂类的组成和比例，制备生物膜模拟物；(2)从蛋白质负载的角度表征其大小和稳定性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bicelles provide an environment wherein proteins can interact with a bilayer structure composed of biologically competent lipids, packaged into a particle size that is sufficiently small to allow for rapid tumbling times in solution for NMR and for high protein concentrations and uniform distributions in the frozen samples used in pulsed dipolar ESR spectroscopy. According to this, it was necessary to engineer suitable bicelles and explore their utility for studies of protein-membrane interactions. We pursued the following specific goal: (1) to prepare bicelles as biological membrane mimetics, based on different compositions and ratios of long chain to short chain lipids; (2) to characterize their size and stability with respect to their loading with protein.