ANTIBODY PRODUCTION AND MOUSE GENOTYPING CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 基因分型核心将设在BBSRB的二楼。目前，核心仍在建立中。我们开发了基于聚合酶链式反应的基因分析方法，并优化了DNA制备程序。所有这些技术都在小规模(约50只动物)上进行了测试。设备、热块、Robcycler PCR机、重复吸管装置和大幅面琼脂糖凝胶电泳仪均已到位。正在编写一套详细的用户说明，以描述要提供什么服务，以及用户需要在样品、聚合酶链式反应引物组和反应条件方面提供什么核心。一则招聘技术员管理该设施的广告已经传阅，申请者正在接受面试。这名技术人员将负责小鼠的基因分型和抗体生产。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The genotyping core will be based on the second floor of the BBSRB. At present the core is still being set up. We have developed the PCR-based methodology for genotype analysis and have optimized the DNA preparation protocols. All of the technologies have been tested on a small scale (~50 animals). The equipment, heat blocks, Robocycler PCR machine, repeating pipette sets, and large format agarose electrophoresis apparati, are in place. A detailed set of user instructions are being written to describe what services are to be provided and what the user needs to supply the core in terms of sample, PCR primer sets, and reaction conditions. An advertisement for a technician to run the facility has been circulated and applicants are being interviewed. The technician will handle the mouse genotyping and antibody production.