ID OF AEROALLERGENIC FUNGI IN RURAL AG, MINE & INDUSTRIAL SOURCES IN BACK HILLS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 空气致敏真菌对人类健康的影响是卫生机构非常感兴趣的，以了解空气致敏真菌如何与健康问题，如特应性哮喘和其他呼吸道疾病。与南达科他州黑山的大平原大气和人类健康中心合作，我们会评估该区空气中真菌分生孢子及菌丝碎片的多样性。我们将通过首先从样本中提取DNA来分析我们的空气样本，然后通过PCR运行DNA，以扩增用于识别物种的DNA区域。稍后，我们将对扩增区域进行测序，并将我们的序列与数据库进行比较，以识别我们的样本。用于物种鉴定的基因组区域包括核核糖体DNA的内部转录间隔区（ITS 1和ITS 2）和核大亚基rDNA的D1-D2可变区。完成后，该项目将有助于更好地了解如何从扰动地球地区产生的空气致敏真菌与呼吸道疾病有关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The impact that aeroallergenic fungi have on people's health is of great interest to health institutions for understanding how aeroallergenic fungi relate to health problems such as atopic asthma and other respiratory illnesses. In conjunction with the Great Plains Center for Atmospheric and Human Health in the Black Hills of S.D., we will assess the diversity of airborne fungal conidia and hyphal fragments in this area. We will analyze our air samples by first extracting DNA from the samples, then running the DNA though PCR in order to amplify the regions of the DNA used to identify species. Later, we will sequence the amplified regions and compare our sequences with databases to identify our samples. The genomic regions that will be used for species identification include the internal transcriber spacer regions (ITS1 and ITS2) of the nuclear ribosomal DNA and the D1-D2 variable region of the large nuclear subunit rDNA. After completion, this project will aid in better understanding of how aeroallergenic fungi produce from areas of disturbed earth relate to respiratory diseases.