ELECTRICALLY HEALING RESPIRATORY EPITHELIAL DAMAGE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 目的1.测定单层培养的猴呼吸道上皮细胞和器官培养的呼吸道上皮细胞的内源性创伤电场。我们将从扑杀的恒河猴(3只雄性或雌性幼年成年猕猴)身上获取呼吸道(气管和支气管组织)。我们将在解剖显微镜下用手术刀制作小的裂隙上皮伤口，并用振动探头测量伤口内源性电流。上皮上无损伤的组织片段将被用作对照。我们还将在单层培养的灵长类呼吸道上皮细胞上制作伤口，并使用振动探头系统来确定内源性伤口产生的电场。 目的2.电促进呼吸道上皮细胞创面愈合。我们将首先在培养中应用电场来灵长类呼吸道上皮细胞，以确定它们对电场的反应。我们还将使用延时视频显微镜监测灵长类呼吸道上皮细胞单层的抓伤愈合情况。我们将像目标1那样在气管内产生小的裂隙上皮伤口，并用药物(氨茶碱、SP 600125、双关木酚、Na(3)VO(4))操纵伤口的电场。用振动探头测量药物治疗后创面的内源性电场。我们还将在单层和组织培养中确定这些药物对伤口愈合的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Aim 1. To measure endogenous wound electric fields in monolayer cultures of respiratory epithelial cells and in organ cultures of respiratory tract epithelia from monkey. We will obtain airway (tracheal and bronchial tissues) from culled Rhesus monkey (3 young adult monkeys either male or female). We will make small slit epithelial wounds with a scalpel under a dissecting microscope and measure the endogenous wound electric currents with a vibrating probe. Segments of tissues without wound on the epithelium will be used as a control. We will also make wounds on monolayer cultures of primate respiratory epithelial cells and use vibrating probe system to determine endogenous wound electric field generated. Aim 2. To enhance wound healing in respiratory epithelia electrically. We will first apply electric fields to primate respiratory tract epithelial cells in culture to determine their response to electric fields. We will also monitor scratch wound healing of primate respiratory tract epithelial cell monolayers with time-lapse video microscopy. We will generate small slit epithelial wounds in trachea as in Aim 1 and manipulate the wound electric fields with pharmacological agents (Aminophylline, SP 600125, dicumarol, Na(3)VO(4)). We will measure the endogenous wound electric fields with the vibrating probe after drug treatment. We will also determine the effects of these drugs on wound healing in monolayer and tissue cultures.