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Degradation of Mislocalized Secretory and Membrane Proteins

Degradation of Mislocalized Secretory and Membrane Proteins
错误定位的分泌蛋白和膜蛋白的降解
批准号：
7968799
负责人：
Ramanujan S Hegde
金额：
$10.24万
依托单位：
EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/7968799
关键词：
Affinity ChromatographyDegradation PathwayElementsFractionationGoalsHomeostasisIn VitroMembrane ProteinsMicrosomesPathway interactionsPreparationProteinsReactionSystemUbiquitinationhigh riskreconstitution

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中文摘要
翻译
在体外重建了对错定位分泌蛋白和膜蛋白的选择性识别和降解。在这个系统中，分泌蛋白和膜蛋白在非易位状态下合成，只需从反应中省略er来源的粗粒体，或者如果包含它们，则使用协转蛋白抑制易位。通过这两种操作，非易位蛋白迅速泛素化，为它们的降解做准备。我们采用经典的分离和亲和纯化方法来鉴定这些非易位蛋白的选择性识别和降解机制。
英文摘要
The selective recognition and degradation of mislocalized secretory and membrane proteins has been reconstituted in vitro. In this system, secretory and membrane proteins are synthesized in their non-translocated state simply by omitting ER-derived rough microsomes from the reaction or, if they are included, by inhibiting translocation using cotransin. With either of these manipulations, non-translocated proteins are rapidly ubiquitinated in preparation for their degradation. We are employing both classical fractionation and affinity purification approaches to identify the machinery for the selective recognition and degradation of these non-translocated proteins.
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会议论文
2014 Protein Transport Across Cell Membrane Gordon Research Conference and Gordon
  • 批准号：
    8643955
  • 项目类别：
  • 资助金额：
    $0.5万
  • 财政年份：
    2014
  • 负责人：
    Ramanujan S Hegde
  • 依托单位：
    GORDON RESEARCH CONFERENCES
Biogenesis Of Secretory And Membrane Proteins
Degradation of Mislocalized Secretory and Membrane Proteins
Chemical Inhibitors of Protein Translocation
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