Laser microdissection for molecular analysis of oral, dental and craniofacial dis

描述(申请人提供)：激光显微解剖(LMD)使区分相关和不相关的细胞或组织成为可能，并使研究人员能够从不同的起始材料中获得均匀的、超纯的样本。研究人员可以有选择地和常规地分析感兴趣的区域，直到单个细胞，以获得相关的、可重复的和特定的结果。伊利诺伊大学芝加哥分校(UIC)现有的多用户LMD系统由医学院病理学系的跨学科病理设施容纳和运营，但需要一个新的系统，因为：1)现有的徕卡AS LMD激光显微切割系统老化，2)大量使用-服务于整个UIC校园。在UIC牙科学院，NIH资助的8个主要项目以及一些小项目需要专用的LMD系统，其中一些项目也是由NIH资助的。这些调查人员在LMD方面经验丰富，但被我们现有系统的局限性所扼杀。最先进的LMD系统将极大地促进这些研究项目的科学进步。主要应用项目包括：1)口腔癌进展和转移的生物标记物鉴定；2)口腔癌和肝癌的DNA损伤检查点通路；3)牙釉质的形成和进化；4)应激损伤微生物清除的分子分析；5)成釉蛋白在牙周发育和牙周膜中的作用；6)上皮-结缔组织的相互作用和瘢痕形成；7)蛇毒在口腔癌发生中的分子机制；8)横纹肌肉瘤的分子分析。

DESCRIPTION (provided by applicant): Laser microdissection (LMD) makes it possible to distinguish between relevant and non-relevant cells or tissues and enables the researcher to obtain homogeneous, ultra-pure samples from heterogeneous starting material. Researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are relevant, reproducible, and specific. The existing multi-user LMD system at University of Illinois Chicago (UIC) is housed and operated by Transdisciplinary Pathology Facility at Department of Pathology, College of Medicine, but a new system is needed, because: 1) the existing Leica AS LMD Laser Microdissection System is aging, and 2) is heavily used - serving the entire UIC campus. A dedicated LMD system is needed for 8 major NIH-funded projects as well as minor projects, some also NIH-funded, at UIC College of Dentistry. These investigators have much experience in LMD, but are stifled by the limitations of our existing system. A state-of-the-art LMD system will greatly facilitate the scientific progress of these research projects. Major user projects include: 1) biomarker identification for oral cancer progression and metastasis, 2) DNA damage checkpoint pathways in oral cancer and liver cancer, 3) dental enamel formation and evolution, 4) molecular analysis of stress impaired microbial clearance, 5) ameloblastin function in periodontal development and the periodontal ligament, 6) epithelial-connective tissue interactions and scar formation, 7) molecular mechanisms of serpins in oral cancer tumorgenesis, 8) molecular analysis of rhabdomyosarcoma.