Acquisition of Microwave-Assisted Automated Peptide Synthesizer

描述(由申请人提供)：申请资金用于购买CEM Liberty全自动微波辅助固相肽合成器及相关配件，以便我们可以在各种合成、蛋白质组和糖蛋白组学应用中使用这项先进技术，重点是糖生物学。这将加强七名目前由NIH资助的研究人员的研究工作，以及一名正在申请NIH的新调查员、一名NSF资助的新调查员和一名新来的正在申请能源部的紧急学者的研究工作。蛋白质糖基化是蛋白质翻译后加工中最常见和最复杂的方式，是影响健康和疾病的重要因素，但糖蛋白的研究往往受到其分子质量和糖链微观异质性的限制。人工合成的糖肽为研究糖蛋白的许多方面提供了强有力的替代试剂。与糖肽合成相关的额外操作严重限制了它们相对于多肽的可用性，一个主要限制因素一直是研究糖蛋白的性质。我们在这一领域一直在使用更费力的常规能力，这一要求的一个关键因素是证明新开发的微波辅助方法被证明对加速糖肽合成特别有益。微波辅助方法在蛋白质水解酶的消化和N-糖链的释放方面也被证明是有益的，满足了几个参与研究的蛋白质组学和糖蛋白研究人员的需要，因此将有助于识别糖蛋白的生物重要方面，并识别需要进一步研究的相关糖肽。该仪器将设在佐治亚大学的复杂碳水化合物研究中心，这是糖生物学研究的卓越中心，并提供一个独特的环境，包括化学、生物化学、分子生物学和细胞生物学专业知识，以便在快速发展的糖生物学领域以最佳方式利用这项技术。 公共卫生相关性：合成和蛋白质组学方面的增强微波辅助技术将被用于推进当前的研究项目，主要集中在研究碳水化合物修饰的蛋白质上。蛋白质糖基化是翻译后修饰的一个非常重要的组成部分，对健康和疾病都有影响，这种仪器将有助于糖蛋白片段的制备和具有生物医学意义的糖蛋白的分析。

DESCRIPTION (provided by applicant): Funds are requested for the acquisition of a CEM Liberty automated microwave-assisted solid phase peptide synthesizer and associated accessories so that we may employ this advanced technology in a variety of synthesis, proteomic, and glycoproteomic applications, with an emphasis on glycobiology. This will enhance the research efforts of seven currently NIH funded investigators, and a new investigator with a pending NIH application, one with NSF funding and a newly arrived Emminent Scholar with a pending DOE application. Protein glycosylation is the most prevalent and complex mode of protein post-translational processing, being an important factor in health and disease, but studying glycoproteins is often limited by their molecular weight and glycan microheterogeneity. Synthetic glycopeptides provide powerful alternative agents for investigating many aspects of glycoproteins. The additional manipulations associated with glycopeptide synthesis have severely limited their availability relative to peptides, a factor that has been a major limitation is studying properties of glycoproteins. We have been working with more laborious conventional capabilities in this area, and a key factor in this request is the demonstration that the newly developed microwave-assisted methodology has proven particularly beneficial for accelerating glycopeptide synthesis. Microwave-assisted approaches have also proven beneficial in proteolytic enzyme digestions and N-glycan release, addressing the needs of several of the participating investigators in porteomics and glycoproteiomcs, and will therefore help identify biologically important aspects of glycorpoteins and identify relevant glycopeptides to be synthesized for further study. This instrumentation would be located in the Complex Carbohydrate Research Center at the University of Georgia which is a preeminent center in glycobiology research, and provides a unique environment encompassing chemistry, biochemistry, molecular biology and cell biology expertise for optimally exploiting the technology in the rapidly developing field of glycobiology. PUBLIC HEALTH RELEVANCE: The enhanced microwave-assisted technologies in synthesis and proteomics will be employed to advance current research projects primarily focusing on investigating carbohydrate modified proteins. Protein glycosylation is a very important component of post-translational modification with impacts in health and disease, and this instrumentation will facilitate preparation of glycoprotein fragments and analysis of glycoproteins of biomedical significance.