Acquisition of a Confocal Microscope for R.M Bock Laboratories

描述（由申请人提供）：本申请申请奥林巴斯FV1000激光扫描共聚焦显微镜的资金。该系统将被安置在威斯康星大学麦迪逊分校的r.m.博克实验室，供该大楼的三个研究实体——分子病毒学研究所（IMV）、分子生物学实验室（LMB）和光学与计算仪器实验室（LOCI）的成员使用。对于研究细胞生物学和开发干细胞、神经、病毒和癌症应用的新型细胞工程方法的研究人员来说，该仪器将是一个有价值的工具。这种共聚焦将用于传统的共聚焦应用，如2D和3D的多标记荧光研究，以及更高级的应用，如光激活、光漂白后的荧光恢复（FRAP）和荧光共振能量转移（FRET）。这种现代研究工具的现场可用性将为现有项目提供更好的设备访问，并使新的研究能够揭示有关细胞过程的关键新信息。

DESCRIPTION (provided by applicant): This proposal requests funds for an Olympus FV1000 laser scanning confocal microscope. This system will be housed in the R.M Bock Laboratories at the University of Wisconsin at Madison and be available for members of the three research entities of this building, the Institute of Molecular Virology (IMV), the Laboratory of Molecular Biology (LMB), and the Laboratory for Optical and Computational Instrumentation (LOCI). This instrument will be a valuable tool to investigators who study cell biology and develop novel cellular engineering approaches for stem, neuro, virus, and cancer applications. This confocal will be used for both conventional confocal applications such as multi-label fluorescence studies in 2D and 3D to more advanced applications such as photoactivation, fluorescence recovery after photobleaching (FRAP), and fluorescence resonance energy transfer (FRET). The availability of this modern research tool on site will facilitate existing projects with better equipment access and enable new studies revealing critical new information about cellular processes. )