Expanding and upgrading a custom built 2-photon microscope for time lapse fluores

描述（由申请人提供）：双光子成像可以提供关于组织内细胞行为的动态信息，包括感染期间和对癌症的反应中的免疫细胞。这些信息可以提供独特的洞察感染期间的事件过程和治疗性治疗在体内的影响。我们现有的双光子显微镜设施目前提供了这一重要的工具，一小群研究人员检查免疫反应的细胞内病原体，但在荧光标记的数量，可以可视化和项目和研究人员的数量，可以容纳的方面是有限的。我们建议通过添加第二台具有5个探测器和更灵活的扫描机制的显微镜以及第二台Ti Sa激光器来扩展我们现有的定制双光子显微镜，以便每个显微镜可以使用2个调谐到不同激发波长的激光器进行操作。我们的建议将提供一个极具成本效益的方式，让更大的灵活性，在成像不同的荧光探针，并容纳更多的用户。

DESCRIPTION (provided by applicant): Two-photon imaging can provide dynamic information about the behavior of cells within tissues including immune cells during infection and in response to cancer. Such information can provide unique insight into the course of events during infection and the impact of therapeutic treatments in vivo. Our existing 2- photon microscopy facility currently provides this important tool to a small group of investigators examining immune responses to intracellular pathogens, but is limited in terms of the number of fluorescent labels that can visualized and the number of projects and investigators that can be accommodated. We propose to expand our existing custom built 2-photon microscope by adding a second microscope with 5 detectors and more flexible scanning mechanism, and a second Ti Sa laser such that each scope can be operated with 2 lasers tuned to different excitation wavelengths. Our proposal will provide an extremely cost effective way to allow greater flexibility in imaging different fluorescent probes and to accommodate a greater number of users.