Illumina Genome Analyzer II

描述（由申请人提供）：DNA序列代表一种单一格式，在这种格式上可以评估广泛的生物现象（例如遗传变异，RNA表达，蛋白质-DNA相互作用，染色体构象等），以进行高通量数据收集。在过去的三年里，大规模并行DNA测序平台已经广泛使用，将DNA测序的成本降低了两个数量级以上，并且基本上将一个主要基因组中心的测序能力交给了能够使用单个这样的仪器的研究人员。这些技术的广泛应用有可能极大地加速基因组学驱动的研究。我们是一个由来自华盛顿大学（UW）三个系（基因组科学，病理学和医学）的11名教师组成的研究小组，他们寻求扩展他们目前在人类和模式生物遗传学，群体遗传学，药物遗传学和系统生物学方面的资助研究，并要求将单个下一代测序仪作为共享资源作为测序成本中心的一部分进行操作。具体而言，申请资金用于购买Illumina基因组分析仪II（“GA-II”）平台，该平台配备了对端模块。该仪器每次运行可以产生超过2.5 gb的酶，分成多达8个文库，每天的速度为1 gb，每兆酶的试剂成本低于5美元。最后，该仪器将受益于DNA测序生产和数据分析方面的重大机构承诺和当地专业知识。

DESCRIPTION (provided by applicant): DNA sequence represents a single format upon which a broad range of biological phenomena (e.g. genetic variation, RNA expression, protein-DNA interactions, chromosome conformation, etc.) can be evaluated for high-throughput data collection. Over the past three years, massively parallel DNA sequencing platforms have become widely available, reducing the cost of DNA sequencing by over two orders of magnitude, and essentially putting the sequencing capacity of a major genome center in the hands of investigators with access to a single such instrument. The widespread application of these technologies has the potential to dramatically accelerate genomics-driven research. We are a group of eleven faculty investigators from three departments (Genome Sciences, Pathology, and Medicine) at the University of Washington (UW), who seek to extend their currently funded research in human and model organism genetics, population genetics, pharmacogenetics, and systems biology, with this request for a single next-generation sequencer to be operated as a shared resource as a part of a sequencing cost center. Specifically, funds are requested to acquire an Illumina Genome Analyzer II ("GA-II") platform, equipped with a paired-end module. The instrument can produce more than ~2.5 gigabases per instrument-run, split across up to 8 libraries, at a rate of 1 Gigabase per day and for reagent costs of less than $5 per megabase. Lastly, this instrument will benefit from significant institutional commitment and local expertise in DNA sequencing production and data analysis.