Leica SP5 Confocal Microscope

描述（由申请人提供）：本提案的目标是为贝勒牙科学院（BCD）光学显微镜核心设施获得徕卡TCS SP5 MP固定阶段（UV- VIS-IR）共聚焦显微镜的资金。该仪器将用于在受控环境中检测从细胞到整个生物体样品的基因表达和蛋白质谱。我们有一台10年的仪器（徕卡SP2），由制造商支持，但不能升级，使用寿命有限。此外，自BCD购买他们的旧仪器以来，共聚焦技术有了很大的改进。在过去的20年里，研究一直是BCD的主要重点。这部分是因为BCD是一个独立的机构。该学院在地理位置上与其附属的医学院分开（德州农工医学院位于得克萨斯州大学城，距离达拉斯约200英里）。BCD拥有一支相对庞大的师资队伍和专门从事教学、生物医学教育和研究的项目。生物医学部现有专任教师22人，博士后15人，在读博士14人。该仪器将支持的项目将扩大对选定细胞类型如何在正常环境中改变基因表达或蛋白质功能的基本理解。这些项目将由高素质的教师和学生解答发育生物学（Svoboda、Opperman、Ruest、Feng、Qin、D’souza）、神经生物学（Kramer、Benson）、免疫学和细胞生物学基础科学问题（Svoboda、Sharma、Benson、Opperman、Feng、Qin）。将这台仪器添加到我们的核心设施的更广泛的影响是，研究生，博士后研究员和教师将有机会使用最先进的仪器。它将提供给所有研究学院，并纳入研究生课程，以促进颅面生物学的基础科学研究。这个核心设施的增加也将加强与其他达拉斯和健康科学中心教师的未来合作，并有助于未来的教师和研究生招聘。

DESCRIPTION (provided by applicant): The goal of this proposal is to obtain funds for a Leica TCS SP5 MP Fixed stage (UV- VIS-IR) confocal microscope for the Baylor College of Dentistry (BCD) light microscopy core facility. This instrument will be used to examine the gene expression and protein profiles of samples from cells to whole organisms in a controlled environment. We have a 10 year old instrument (Leica SP2) that is supported by manufacturer, but it cannot be upgraded and it has a limited lifetime. In addition, confocal technology has improved greatly since BCD purchased their older instrument. Research has been a major emphasis at BCD over the past 20 years. In part, this is because BCD is a stand-alone institution. The college is geographically separated from its affiliated medical school (Texas A & M College of Medicine, College Station, TX is about 200 miles from Dallas). BCD maintains a relatively large group of faculty and programs that are devoted teaching, biomedical education and research. There are 22 full time faculty, 15 postdoctoral fellows and 14 Ph.D. students in the Department of Biomedical Sciences working on different research projects. The projects that will be supported by this instrument will expand the basic understanding of how selected cell types change gene expression or protein functions within their normal environment. These projects will address developmental biology (Svoboda, Opperman, Ruest, Feng, Qin, D'Souza), neurobiology (Kramer, Benson), immunology and cell biology basic science questions (Svoboda, Sharma, Benson, Opperman, Feng, Qin) by highly qualified faculty and their students. The broader impact of adding this instrument to our core facilities is that graduate students, postdoctoral fellows, and faculty will have access to a state of the art instrument. It will be available to all research faculties and incorporated into graduate courses to promote basic science research in craniofacial biology. The addition of this core facility will also enhance future collaborations with faculty from other Dallas and health science center faculty and aid in future faculty and graduate student recruitment.