Typhoon 9140

描述(申请人提供)：凝胶和斑点的成像是生物医学研究的常规部分。在这项提案中，需要资金购买台风9410凝胶和斑点成像系统，以更换和升级现有的Storm 860成像系统，该成像系统是用克雷顿大学医学院于2000年提供的内部资金购置的。与Storm 860相比，该设备具有更高的灵敏度，并提供多种检测方式，包括用于对放射性标记的核酸和蛋白质进行成像的存储荧光粉技术，以及使用多个波长的直接荧光和化学荧光成像能力，从而能够同时检测多个探针，用于免疫印迹和微阵列分析等应用。这些不同的检测模式将被用来支持和加强克雷顿大学正在进行的由NIH资助的研究，这些领域包括Pron发病机制、皮肤癌变、抗原受体基因多样化、眼腺发育和调控RNA。该仪器还将改善Creighton研究社区可用的整体研究基础设施，以开发新的与健康相关的研究计划。此外，收购台风9410将使现有的Storm 860转移到文理学院化学和生物系，以提升本科生的研究和教学机会，这将促进下一代科学家的发展和培养。

DESCRIPTION (provided by applicant): The imaging of gels and blots is a routine part of biomedical research. In this proposal, funds are requested to purchase a Typhoon 9410 gel and blot imaging system to replace and upgrade an existing Storm 860 imaging system acquired using intramural funds provided by the Medical School at Creighton University in 2000. This equipment provides superior sensitivity compared to the Storm 860 and provides several detection modalities, including storage phosphor technology to image radioactively labeled nucleic acids and proteins, and direct fluorescent and chemifluorescent imaging capability using several wavelengths that enables simultaneous detection of multiple probes for applications such as immunoblotting and microarray analysis. These various detection modalities will be used to support and enhance ongoing NIH-funded research at Creighton University in areas such as prion pathogenesis, skin carcinogenesis, antigen receptor gene diversification, ocular gland development, and regulatory RNAs. This instrumentation will also improve the overall research infrastructure available to Creighton's research community to develop new health-related research programs. In addition, the acquisition of the Typhoon 9410 will enable the existing Storm 860 to be transferred to the College of Arts and Sciences Departments of Chemistry and Biology to enhance undergraduate research and teaching opportunities, which will promote the development and training of the next generation of scientists.