BIAcore T100 for research and drug discovery in Rochester

描述（由申请人提供）：本共享仪器提案旨在为罗切斯特大学购买最先进的BIAcore T100仪器提供资金。在罗切斯特地区没有可与T100相比的手术工具。一个内部Reichert SR7000仪器已经导致一个社区，需要一个自动化，灵敏，灵活的BIAcore T100加速研究和药物发现。八个主要用户要求该仪器用于研究RNA剪接、出血性疾病、信号转导、DNA复制、染色质组织和HIV-1复制等过程，以及表征潜在的治疗性低分子量化合物。BIAcore T100由罗切斯特大学战略计划投资的几个中心提供支持。因此，通过这些中心和通过在安装仪器之前计划的技术研讨会的广告，预计将出现许多新用户。像罗切斯特大学的许多共享设施一样，我们计划将BIAcore T100提供给任何需要该技术进行研究的人。所有用户都需要接受BIAcore T100正确使用和维护的特定培训。一个由四名成员组成的咨询委员会将批准新用户并解决争议。我们交叉列出的生物物理技术研究生课程也将培养学生对BIAcore数据的理论和批判性分析，并通过BIAcore T100软件的实践经验得到加强。BIAcore T100将立即提高罗切斯特地区的生产力，并显著扩大研究和药物发现能力。

DESCRIPTION (provided by applicant): This shared instrumentation proposal seeks funds to purchase a state-of-the-art BIAcore T100 instrument for the University of Rochester. No instrument comparable to the T100 is available in the Rochester area. An in- house Reichert SR7000 instrument has led to a community that needs an automated, sensitive, and flexible BIAcore T100 for expedited research and drug discovery. Eight primary users request the instrument for investigating processes such as RNA splicing, bleeding disorders, signal transduction, DNA replication, chromatin organization, and HIV-1 replication, as well as to characterize potentially therapeutic, low molecular weight compounds. The BIAcore T100 is supported by several Centers targeted for investment by the Strategic Plan for the University of Rochester. Accordingly, many new users are expected to emerge both through these Centers and through advertisement of a technical seminar planned prior to instrument installation. Like many shared facilities at the University of Rochester, we plan to make the BIAcore T100 available to anyone who needs the technique for their research. All users are required to receive specific training in the appropriate use and maintenance of the BIAcore T100. A four-member advisory committee will approve new users and settle disputes. Our cross-listed graduate course in biophysical techniques will also train students in the theory and critical analysis of BIAcore data, and in turn be strengthened by hands-on experience with the BIAcore T100 software. The BIAcore T100 would immediately increase the productivity of and significantly broaden research and drug discovery capabilities in the Rochester area.