Administrative Core

提供设施 我们的五个实验室足以按要求执行具体的五个项目。此外，还有一个 我们共享的集体设施的数量。这些实验室有一个媒体厨房，面积约为600平方米。英国《金融时报》的 专门用于制备果蝇培养基板和蠕虫板的空间。人造海水也是由 这个设施。厨房目前有自己的高压灭菌器、办公室和存储设施。此外，实验室 在BASINE大楼的地下室共用一个特殊的空间(“坑”：大约1000平方米。英国《金融时报》)其中 几个苍蝇孵化器被安置在里面，用于监测昼夜节律和热量活动。光线紧绷， 这个空间的恒温性质使它在恒定的黑暗中以及 控制光暗和温度条件。这是许多行为监测进行的地方。 除此之外，还有一个60平方英尺的房间。加里提/格里菲斯的哈里斯房间温湿度可控 用于冷漠行为和学习的空间。 所有实验室都可以使用可用的部门资源，包括一台磷光成像仪设备，Conocus 设施、微阵列/FACS设施等。共焦实验室由所有人指导和大量使用 实验室。除此之外，还有一个共享成像设备，它有一个双光子显微镜和 两台实时荧光显微镜。Fly实验室共用一个注射设施(320平方英尺)。英国《金融时报》)有两个人 用于产生转基因果蝇的显微注射装置。微阵列/流式细胞仪设施是共享资源 两栋楼外。这些设施包括Affymetrix微阵列处理仪器(杂交 一台安捷伦2100生物分析仪系统和一台BD FACSAria流式细胞仪。 我们有一个核磁共振设施，以及一个新的MALDI和多肽分析中心。结构生物学小组位于 Brandeis拥有并运营一家一流的最先进的电子显微镜设施。一家机械车间和 生物系有电子商店，科学图书馆就在隔壁 大楼。 行政安排 在过去的二十三年里，这笔赠款的PI一直是罗斯巴什先生。霍尔和怀特 在过去五年的拨款中，重要的领导角色是将火炬传递给马德和格里菲斯。 Rosbash主要负责赠款管理，Rosbash和Marder都负责 全面科学领导，把好质量关。格里菲斯负责媒体厨房和其他方面的工作 核心，包括监督显微镜设施及其技术人员。罗斯巴什的助理/实验室经理 希瑟·费尔顿，拥有分子生物学博士学位，也是生物系主任 芭芭拉·莱特森为拨款相关事宜提供行政支持。费尔顿是HHMI的一名员工 莱特森100%由布兰迪斯大学支付。这五名PI都是Brandeis生物系的成员， 从管理的角度来看，这是他们的主要从属关系，也是沃伦综合中心的主要从属关系 系统。自从取消了这笔赠款中的任何工资以来，罗斯巴什已经从HHMI那里获得了100%的工资 那一次。该小组中的大多数学生和博士后都会参加由 生物系、生物化学系和神经科学界。也有 计划项目每月研讨会，以促进五个实验室之间的更紧密联系。苍蝇团体举行了一场 每月的“Flyfest”，内部和外部演讲者都会在会上展示他们的作品。

FACILITIES AVAILABLE Our five laboratories are adequate to carry out the specific five projects as requested. There are in addition a number of collective facilities, which we share. The labs have a media kitchen with approximately 600 sq. ft. of space dedicated to preparation of Drosophila media and worm plates. Artificial seawater is also produced by this facility. The kitchen currently contains its own autoclave, office, and storage facilities. In addition, the labs share a special space in the basement of the Bassine building ("the pit": approximately 1000 sq. ft.) in which several fly incubators are housed that are used to monitor circadian and thermal activity. The light tight and constant temperature nature of this space makes it optimal for entrainment in constant dark as well as controlled light-dark and temperature conditions. This is where much of the behavioral monitoring takes place. In addition to this there is a 60 sq ft. temperature and humidity controlled Harris room in the Garrity/Griffith space that is used for cold behavior and learning. All of the labs have access to available departmental resources including a phosphorimager facility, confocal facility, microarray/FACS facility, etc. The Confocal laboratory is directed by and used heavily by all laboratories. In addition to this there is also a shared Imaging Facility that has a two-photon microscope and two real-time epifluorescence microscopes. The Fly labs share an injection facility (320 sq. ft.) with two microinjection setups for the generation of transgenic flies. The Microarray/FACS Facility are shared resources 2 buildings away. These facilities contain Affymetrix microarray processing instrumentation (hybridization oven, fluidics station, and scanner), an Agilent 2100 Bioanalyzer system, and a BD FACSAria Flow Cytometer. We have a NMR facility, and a new MALDI and peptide analysis center. The structural biology group at Brandeis owns and operates a superb state-of-the art electron microscope facility. A machine shop and electronics shop are available to the Biology Department and the Science library is located in the adjacent building. ADMINISTRATIVE ARRANGEMENTS The PI of this grant is and has been M. Rosbash for the past twenty-three years. Hall and White who played important leadership roles during the past five years of the grant are passing the torch to Marder and Griffith. Rosbash is principally in charge of grant management and both Rosbash and Marder are responsible for overall scientific leadership and quality control. Griffith is in charge of the media kitchen and other aspects of the Core, including oversight of the microscope facility and its technician. Rosbash's assistant/lab manager Heather Felton, who holds a doctorate in Molecular Biology, as well as the Biology Department administrator Barbara Wrightson provide administrative support for grant-related matters. Felton is an HHMI employee and Wrightson is 100% paid by Brandeis University. All five Pis are members of the Brandeis Biology Department, which is their primary affiliation from an administrative standpoint, and of the Volen Center for Complex Systems. Rosbash has received 100% salary from HHMI since 1989 eliminating any salary from this grant at that time. Most students and post-docs in the group attend the ample seminars and journal clubs hosted by the Biology Department, the Biochemistry Department and the Neuroscience community. There are also Program Project monthly seminars to foster closer contact between the five laboratories. The fly groups hold a monthly "FlyFest" at which both inside and outside speakers present their work.