CHARACTERIZATION OF A MORAXELLA SPECIES THAT CAUSES EPSTAXIS IN MACAQUES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 莫拉氏菌属的成员是革兰氏阴性需氧菌，已从各种哺乳动物宿主中分离出来。在一项对恒河猴鼻咽部细菌的调查中，发现了莫拉氏菌属的分离株，鉴定为M。在大多数表现出鼻出血或“血鼻综合征”的动物中观察到卡他。“这次调查中的几个分离株在杜兰国家灵长类动物研究中心冷冻保存。还从美国陆军传染病医学研究所的上位性食蟹猴中获得了一种分离株。 根据恒河猴分离殖民地与人M.卡他分离株，我们假设，非人灵长类动物莫拉菌可能是独一无二的。通过多个参数重新评估这些分离株。尽管在细胞和菌落形态上存在差异，但通过每项生化试验，恒河猴分离株与M。而不是其他人类莫拉氏菌亚种。通过Vitek 2 compact（圣刘易斯，密苏里州）对食蟹猴分离株进行的生化分析显示，其属于莫拉氏菌属，但无法区分种属。 然而，16 S核糖体RNA基因的测序，从四个代表性的恒河猴分离株和食蟹猴分离株显示最接近的同源性，与人类呼吸道居民，与90.16%的同一性。 为了研究恒河猴作为M.卡他，总共8只动物接种该菌株的人分离株。 只有一只动物被定殖并表现出疾病，而四只猕猴中的四只在接种恒河猴莫拉氏菌分离株后成为上位性。因此，这种鼻咽莫拉氏菌属是一种以前没有特征的非人灵长类动物的独特病原体。这不是M。粘膜炎。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Members of the Moraxella genus are gram-negative, aerobic bacteria that have been isolated from a variety of mammalian hosts. During a survey of bacteria that colonize the nasopharynx of rhesus macaques, isolates of the Moraxella genus, identified as M. catarrhalis, were observed in the majority of animals with demonstrated epistaxis, or "bloody nose syndrome." Several isolates from this survey were cryopreserved at the Tulane National Primate Research Center. One isolate was also obtained from an epistatic cynomolgus macaque at the U.S. Army Medical Research Institute of Infectious Diseases. Based on differences between the colony and gram stain morphology of rhesus isolates compared to that of human M. catarrhalis isolates, we hypothesized that the nonhuman primate Moraxella might be unique. These isolates were reassessed by multiple parameters. Despite differences in cell and colony morphology, the rhesus isolates, by each biochemical test, were identical to M. catarrhalis and not other human Moraxella subspecies. Biochemical analysis of the cynomolgus isolate by Vitek 2 compact (St Louis, Mo) revealed that it belonged to a Moraxella group, but could not differentiate between species. However, sequencing of the 16S ribosomal RNA gene from four representative rhesus isolates and the cynomolgus isolate showed closest homology to Moraxella lincolnii, a human respiratory tract inhabitant, with 90.16% identity. In order to examine rhesus macaques as potential hosts for M. catarrhalis, a total of eight animals were inoculated with human isolates of this strain. Only one of the animals was colonized and showed disease, whereas four of four macaques became epistatic after inoculation with the rhesus Moraxella isolate. Thus, this nasopharyngeal Moraxella species is a previously uncharacterized, distinct pathogen of nonhuman primates. It is not M. catarrhalis.