PROOF OF CONCEPT FOR CARD-BASED CD4 CELL COUNTING

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 第二阶段SBIR应用将继续开发一种廉价的护理点卡/阅读器系统，用于监测艾滋病毒感染者的CD 4+细胞，供资源贫乏地区使用。 该技术使用薄膜层压微流体盒来收集、计数和储存血液样本，同时储存所需的检测试剂并从台式单元注入盒中。其目的是系统地开发和故障排除设备，最终验证其读数与标准流式细胞术相比，使用来自人类受试者的全血以小体积计数CD 4+细胞，每次测试不到1.50美元。我们已经证明，包被有抗体的特定大小的荧光珠可以特异性标记人CD 4细胞（单核细胞和T细胞），并且这些细胞在盒中以及在标准流式细胞仪中被检测和区分。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This phase II SBIR application will continue the development of an inexpensive point-of-care card/reader system for monitoring CD4+ cells in HIV-infected subjects, for use in resource poor areas. The technology uses a thin-film laminated microfluidic cassette to collect, count and store the blood sample while the required detecting reagents are stored and injected into the cassettes from the bench top unit. The aims are designed to systematically develop and troubleshoot the device, ultimately validating its readings comparing with standard flow cytometry using whole blood from human subjects to enumerate CD4+ cells in small volumes for less than $1.50 per test. We have shown that fluorescent beads of a specific size coated with antibody can specifically label human CD4 cells (both monocytes and T cells) and that these cells are detected and discriminated in the cassette, as well as in the standard flow cytometer.