IN VIVO SAFETY AND EFFICACY OF CAP FILM AND MICROBICIDE COMBINATIONS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 背景：我们建议建立单独的MIV-160对含有HIV-1逆转录酶基因的猿人免疫缺陷病毒(SHIV-RT)的效力。六只动物被用来做这个实验。方法：对6只成年雌性印度猕猴阴道内注射MIV-160,500M/ml羟甲基纤维素凝胶溶液3ml。给药半小时后，每只动物阴道内注射1ml大剂量10000 TCID50的SHV-RT。同样，每只动物阴道内注射2毫升的MIV-160。Shiv-RT挑战后30分钟。 结果/讨论：所有动物均被感染。MIV-160未能阻止该病毒的传播。所有动物都有很高的病毒设定点，这表明MIV-160不会改变也不会减弱SIV-RT。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: We proposed to establish the efficacy MIV-160 alone against simian human immunodeficiency virus containing the reverse transcriptase gene of HIV-1 (SHIV-RT). Six animals were used for this experiment. Methods: Three (3) ml of MIV-160, 500¿M/ml in hydroxymethycellulose (HEC) gel was applied vaginal into 6 adult Indian female macaques. Half -hour post gel application, each animal was challenged vaginally with 1 ml high dose 10000 TCID50 of SHIV-RT. Again, 2 ml each of MIV-160 was applied vaginally in the respective animals. 30 minutes post SHIV-RT challenge. Results/Discussion: All animals were infected. MIV-160 failed to impede transmission of the impute virus. All animals had high viral set points which indicate that MIV-160 did not alter nor attenuate SHIV-RT.