Cell and Tissue Imaging Core Facility

癌症中心“细胞和组织成像核心设施”（以前的“共聚焦核心设施”） 已经进入了第六个年头。它的使命是由癌症中心执行委员会制定的， 提供先进的细胞和组织成像技术、服务和科学咨询， 科学互动和提高癌症中心研究人员的科学生产力。该设施 最初是与Doheny眼科研究所合作开发的;合作机构共同购买了 第五代共聚焦显微镜，并将仪器安装在现有的电子显微镜成像中 与癌症中心相邻。该设施由癌症中心和Doheny联合管理。 眼科研究所。该共享设施的2，000平方英尺实验室空间由Doheny Eye提供 研究所在没有成本的癌症中心。因此，该设施50%由癌症中心使用，50% Doheny眼科研究所本提案中要求的所有资金均用于与癌症直接相关的费用 中心50%的使用权。 这个共享资源设施的主要用户是癌症中心的研究人员， 资助的项目。该设施也向需要其设施的其他癌症中心成员开放 开发试点项目数据。自成立以来，该基金为癌症中心提供了 会员可轻松获得“最先进”的细胞和组织成像设备及技术支持。 共聚焦显微镜的日间使用仍然很重（90%的可用时间利用率）;大约 其中50%的使用来自癌症中心会员（与50：50使用安排一致， Doheny眼科研究所）。重要的是，癌症中心提供的成像设备和服务范围 成员已扩大（超越激光扫描共聚焦/多光子显微镜），包括全面访问 透射电子显微镜（TEM）、扫描电子显微镜（SEM）、数码光和荧光 显微镜、荧光和明场激光捕获显微切割、薄切片、冷冻切片和 嵌入程序和计算机辅助图形。我们不断升级我们的成像软件 并建议和指导癌症中心的研究人员在新的方法和协议，以提高 他们的细胞和组织成像能力。

The Cancer Center "Cell and Tissue Imaging Core Facility" (previously the "Confocal Core Facility") is entering its sixth year of operation. Its mission, as developed by the Cancer Center Executive Committee, is to provide advanced cell and tissue imaging technology, services, and scientific consultation that facilitate scientific interaction and enhance scientific productivity of Cancer Center investigators. The Facility was originally developed as a partnership with the Doheny Eye Institute; the partner institutes jointly purchased a 5th generation confocal microscope and housed the instrument in an existing electron microscopy imaging facility adjacent to the Cancer Center. The Facility is jointly managed by the Cancer Center and the Doheny Eye Institute. The 2,000 square foot laboratory space for this shared facility is provided by the Doheny Eye Institute at no cost to the Cancer Center. The Facility is therefore used 50% by the Cancer Center and 50% by Doheny Eye Institute. All funds requested in this proposal are for costs directly related to the Cancer Center's 50% use of the facility. The primary users of this shared resource facility are Cancer Center investigators with peerreviewed, funded projects. The Facility is also open to other Cancer Center members who need its facilities to develop pilot project data. Since its establishment, the Facility has provided the Cancer Center membership with easy access to "state-of-the-art" cell and tissue imaging equipment and technical support. Daytime usage of the confocal microscope remains heavy (90% utilization of available hours); approximately 50% of this usage comes from Cancer Center members (consistent with the 50:50 use arrangement with Doheny Eye Institute). Importantly, the range of imaging equipment and services available to Cancer Center members has expanded (beyond laser scanning confocal/multiphoton microscopy) to include full access to transmission electron microscopy (TEM), scanning electron microscopy (SEM), digital light and fluorescence microscopy, fluorescence and bright field laser capture microdissection, thin sectioning, cryo-sectioning and embedding procedures, and computer aided graphics. We are continuously upgrading our imaging software and advising and instructing Cancer Center investigators in novel methodologies and protocols to enhance their cell and tissue imaging capability.