Acquisition of a 600 MHz NMR cryoprobe and 600 MHz spectrometer console upgrade

描述(由申请人提供)：请提供支持以获取高灵敏度600 MHz三重共振(1H，13C，15N)TCI低温探头，并将蒙大拿州立大学(MSU)现有的600 MHz(1H Larmor频率)核磁共振光谱仪控制台升级到更自动化和高通量的数据收集平台(Bruker Avance IIITM)。该仪器将由来自密歇根州立大学化学和生物化学系、兽医分子生物学系、化学和生化工程系、生物膜工程中心、细胞生物学和神经科学系以及NIH落基山实验室的不同研究人员使用。目前，密歇根州立大学结构生物学和化学核磁共振设施中的所有溶液核磁共振光谱仪都没有配备冷冻探头，所要求的仪器和光谱仪控制台硬件的升级将是校园内的第一次。所要求的仪器升级将提高MSU 600 MHz溶液核磁共振光谱仪的灵敏度并最大限度地提高吞吐量。它将对成功完成具有挑战性的基于核磁共振的结构生物学项目和扩展正在校园内开发的核磁共振代谢组学研究项目做出重大贡献。所要求的仪器实现的灵敏度大大提高，将允许对具有挑战性的生物样品进行实验和研究，目前由于样品溶解度有限、样品稳定性有限或仪器时间有限，这些实验和研究是不可能的。控制台升级将允许自动垫片和性能优化，并将更有效地使用最近购买的自动样品加载系统(Bruker SampleJetTM)。控制台升级和自动样品加载系统的结合将使600 MHz核磁共振仪得到更有效的全天候利用。低温探头和升级的控制台将极大地促进我们现有核磁共振代谢组学能力的扩展(包括用于代谢物图谱分析的ChenomxTM和AMIXTM软件)。核磁共振结构生物学和代谢组学基础设施的增强是许多潜在用户所希望的，并支持由NIH资助的新的P20细胞机制和系统生物学分析中心的发展。密歇根州立大学的DRX 600核磁共振仪器的使用相当频繁，但所要求的升级提供的增强的灵敏度和性能将使该仪器能够完成更多的工作。所需仪器的增加将为密歇根州立大学博兹曼分校的广泛研究人员提供显著增强的生物医学研究能力。所要求的仪器还将为转译生物医学研究的新研究方向提供机会，将支持密歇根州立大学扩展的系统生物学方法，并将有助于培训和雇用大量研究生、博士后研究员和研究人员。 公共卫生相关性：我们请求美国国立卫生研究院提供支持，以增强蒙大拿州立大学的核磁共振生物医学研究能力。所要求的仪器将增强广大科学家的研究能力，这些科学家参与更好地了解铁稳态、宿主与病原体相互作用、病毒感染、阿尔茨海默氏症、糖尿病、艾滋病和其他重要人类疾病的分子基础。

DESCRIPTION (provided by applicant): Support is requested to acquire a high-sensitivity 600 MHz triple resonance (1H, 13C, 15N) TCI cryoprobe and to upgrade an existing 600 MHz (1H Larmor frequency) NMR spectrometer console at Montana State University (MSU) to a more automated and high-throughput data collection platform (Bruker Avance IIITM). This instrumentation will be used by a diverse group of researchers from MSU Departments of Chemistry and Biochemistry, Veterinary Molecular Biology, Chemical and Biochemical Engineering, the Center for Biofilm Engineering, the Department of Cell Biology and Neuroscience, and the NIH Rocky Mountain Laboratory. Currently, none of the solution NMR spectrometers in the MSU Structural Biology and Chemistry NMR Facility are equipped with a cryoprobe, and the requested instrumentation and upgrade of the spectrometer console hardware will be the first of its kind on campus. The requested instrumentation upgrades will enhance sensitivity and maximize throughput of MSU 600 MHz solution NMR spectrometer. It will contribute significantly to the successful completion of challenging NMR-based structural biology projects and the expansion of NMR metabolomics research programs that are being developed on campus. The very substantially enhanced sensitivity achieved with the requested instrumentation will allow acquisition of experiments and studies on challenging biological samples, which are currently not possible because of limited sample solubility, limited sample stability, or limited instrument time availability. The console upgrade will allow automated shimming and performance optimization, and will provide much more efficient use of the recently purchased automatic sample loading system (Bruker SampleJetTM). The combination of the console upgrade and the automatic sample loading system will allow more effective 24/7 utilization of the 600 MHz NMR instrument. The cryoprobe and upgraded console will greatly facilitate the expansion of our existing NMR metabolomics capabilities (which include ChenomxTM and AMIXTM software for metabolite profiling analysis). Enhancement of NMR structural biology and metabolomics infrastructure is desired by a number of prospective users and supports the development of a new NIH-funded P20 Center for the Analysis of Cellular Mechanisms and Systems Biology. MSU's DRX 600 NMR instrument is used quite heavily, but the enhanced sensitivity and performance provided by the requested upgrades will allow this instrument to accomplish much more. The addition of the requested instrumentation will leverage significantly enhanced biomedical research capabilities for a broad group of researchers on the MSU-Bozeman campus. The requested instrumentation will also provide opportunities for new research directions in translational biomedical research, will support expanded systems biology approaches at MSU, and will contribute to the training and employment of numerous graduate students, post-doctoral fellows, and research staff. PUBLIC HEALTH RELEVANCE: We request support from the NIH to enhance the nuclear magnetic resonance (NMR) biomedical research capabilities of Montana State University. The requested instrumentation will enhance the research capabilities of a broad group of scientists involved in better understanding the molecular basis of iron homeostasis, host-pathogen interactions, viral infections, Alzheimer's, diabetes, AIDS, and other important human diseases.